BASIC FIBROBLAST GROWTH-FACTOR ENHANCES MYOCARDIAL COLLATERAL FLOW IN A CANINE MODEL
BASIC FIBROBLAST GROWTH-FACTOR ENHANCES MYOCARDIAL COLLATERAL FLOW IN A CANINE MODEL
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DOI：
10.1152/ajpheart.1994.266.4.h1588
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发表时间：
1994-04-01
影响因子：
--
通讯作者：
EPSTEIN, SE
中科院分区：
文献类型：
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作者：
UNGER, EF;BANAI, S;EPSTEIN, SE
碱性成纤维细胞生长因子 (FGF) 是一种多功能肽，可能在与冠状动脉侧枝形成和心肌梗塞愈合相关的血管生成中发挥不可或缺的作用。我们试图确定外源性施用碱性 FGF 对缺血心肌侧支血流的影响。 Ameroid 收缩器被用来导致狗的左冠状动脉回旋支逐渐闭塞。碱性 FGF（110 μg，n = 9）或生理盐水（n = 12）每日推注直接注射到侧支依赖区，从放置 Ameroid 后 10 天开始，持续 28 天。每周评估侧支血流，作为最大药物诱导的冠状血管舒张期间侧支血流与正常区（CZ/NZ）血流的比率。作为时间的函数，治疗犬和对照犬的 CZ/NZ 均有所增加；然而，到治疗第二周时，碱性 FGF 治疗犬的跨壁侧支血流显着超过对照犬。治疗组和对照组的最终 CZ/NZ 血流量比分别为 0.49 +/- 0.05 和 0.35 +/- 0.02（平均值 +/- SE，P = 0.0002）。碱性 FGF 治疗还与 CZ 内分布血管的数量密度和内皮细胞 DNA 合成的显着增加相关。我们还发现碱性 FGF 作为冠状血管扩张剂具有急性作用。因此，外源性施用碱性 FGF 可以增强患有继发于单血管冠状动脉闭塞的心肌缺血的狗的最大侧支血流量，这种作用可能是通过肽的直接血管生成作用介导的，尽管其急性血管舒张作用也可能发挥作用。
Basic fibroblast growth factor (FGF) is a multifunctional peptide that may play an integral role in angiogenesis associated with coronary collateral formation and myocardial infarct healing. We sought to determine the effects of exogenously administered basic FGF on collateral blood flow to ischemic myocardium. Ameroid constrictors were used to cause gradual occlusion of the left circumflex coronary artery in dogs. Basic FGF (110 mu g, n = 9) or saline (n = 12) was given as a daily bolus injection directly into the collateral-dependent zone, beginning 10 days after placement of the Ameroid and continuing for 28 days. Collateral flow was assessed weekly as the ratio of collateral to normal zone (CZ/NZ) blood flow during maximal pharmacologically induced coronary vasodilation. The CZ/NZ increased in both treated and control dogs as a function of time; however, transmural collateral flow in basic FGF-treated dogs significantly exceeded that of control dogs by the second week of treatment. Final CZ/NZ blood flow ratios were 0.49 +/- 0.05 and 0.35 +/- 0.02 in the treated and control groups, respectively (means +/- SE, P = 0.0002). Treatment with basic FGF was also associated with significant increases in the numerical density of distribution vessels and endothelial cell DNA synthesis within the CZ. We also found that basic FGF had acute effects as a coronary vasodilator. Thus exogenous administration of basic FGF enhances maximal collateral blood flow in dogs with myocardial ischemia secondary to single-vessel coronary occlusion, an effect that is likely mediated through the direct angiogenic effects of the peptide, although its acute vasodilatory effects may also play a role.