THE PROTECTIVE ACTION OF A SPECIFIC ENZYME AGAINST TYPE III PNEUMOCOCCUS INFECTION IN MICE.
THE PROTECTIVE ACTION OF A SPECIFIC ENZYME AGAINST TYPE III PNEUMOCOCCUS INFECTION IN MICE.
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DOI：
10.1084/jem.54.1.73
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发表时间：
1931-06-30
期刊：
影响因子：
--
通讯作者：
Dubos R
中科院分区：
文献类型：
--
作者：
Avery OT;Dubos R
在体外分解纯化的III型肺炎球菌荚膜多糖的细菌酶也破坏在培养基和动物体内生长的活生物体的荚膜。这种酶的有效制剂可以保护小鼠免受致命的III型肺炎球菌的感染。保护作用是特定类型的。特定酶的保护活性被热（70°C）破坏。10分钟）。这种酶在注射到正常小鼠体内后24至48小时仍保持有效浓度。已发现该酶对治疗时已建立的感染的结果产生有利影响。已发现在酶的体外活性与其在动物体内的保护力之间存在一定的关系。保护作用的机制进行了讨论，特别是参考由酶和宿主的吞噬反应的细菌的解封之间的关系。
The bacterial enzyme which decomposes the purified capsular polysaccharide of Type III Pneumococcus in vitro also destroys the capsules of the living organisms growing in media and in the animal body. Potent preparations of this same enzyme protect mice against infection with virulent Type III Pneumococcus. The protective action is type-specific. The protective activity of the specific enzyme is destroyed by heat (70°C. for 10 minutes). The enzyme remains in an effective concentration 24 to 48 hours after its injection into normal mice. The enzyme has been found to exert a favorable influence on the outcome of an infection already established at the time of treatment. A definite relationship has been found to exist between the activity of the enzyme in vitro and its protective power in the animal body. The mechanism of the protective action is discussed with special reference to the relation between the decapsulation of the bacteria by the enzyme and the phagocytic response of the host.