Studies of in vivo recruitment and activation of cytotoxic lymphocytes using a gelatin-sponge model of concomitant tumor immunity.
Studies of in vivo recruitment and activation of cytotoxic lymphocytes using a gelatin-sponge model of concomitant tumor immunity.
复制标题
使用伴随肿瘤免疫的明胶海绵模型研究细胞毒性淋巴细胞的体内募集和激活。
DOI：
10.1002/ijc.2910620411
复制
发表时间：
1995
影响因子：
6.4
通讯作者：
Akporiaye,ET
中科院分区：
文献类型：
--
作者：
Park,JA;Brown,RA;Kurt,RA;Akporiaye,ET
We have earlier described a sponge model of concomitant tumor immunity that permits the capture and isolation of effector T lymphocytes that mediate the rejection of a secondary EMT6 tumor implant In this study, we have employed the sponge model to study lymphocyte homing andin situactivation during the development of concomitant tumor immunity. Our results demonstrate that EMT6‐specific CTL in animals bearing primary EMT6 tumors home preferentially to sponges implanted with EMT6 tumor cells, as compared with contralateral sponges lacking tumor cells or sponges injected with antigenically distinct 168 tumor cells. We further show that recruitment is selective and is not in response to a foreign‐body reaction to the sponge. In addition, we show that EMT6‐specific CTL were recovered from sponges injected either with intact EMT6 tumor cells or with a mixture of EMT6‐derived membranes and supernatant. In contrast, cells accumulating in sponges injected with membranes or supernatant alone were not cytolytic. Thus, maximal recruitment, retention, and activation of CTL precursors require putative chemo‐attractive factors secreted by tumor cells as well as interaction with tumor antigen. © 1995 Wiley‐Liss, Inc.
