Protection against L3 induced Brugia malayi infection in Mastomys coucha pre-immunized with BmAFII fraction of the filarial adult worm
Protection against L3 induced Brugia malayi infection in Mastomys coucha pre-immunized with BmAFII fraction of the filarial adult worm
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DOI：
10.1016/j.vaccine.2006.05.003
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发表时间：
2006-07-26
期刊：
影响因子：
5.5
通讯作者：
Murthy, P. K.
中科院分区：
文献类型：
--
作者：
Dixit, S.;Gaur, R. L.;Murthy, P. K.
本研究旨在研究马来布鲁吉虫成虫提取物的BmAFII （Sephadex G-200洗脱部分）对传染性幼虫（L-3）诱导的马来布鲁吉虫感染在coucha Mastomys中建立的保护作用，并描述宿主诱导的免疫反应。用BmAFII免疫健康雄性coucha，然后接种马来双球菌L-3。特异性IgG和细胞介导的免疫反应（细胞增殖），包括肿瘤坏死因子- α (tnf - α)、干扰素- γ (ifn - γ)、转化生长因子- β (tgf - β)和一氧化氮（NO）的释放，由体外BmAFII或脂多糖(LPS)/魔豆蛋白A （Con A）刺激的宿主细胞。BmAFII免疫使成虫回收率降低85.7% (P < 0.001)，使微丝虫病发生率降低77 ~ 95% （P < 0.05 ~ 0.01）。单独免疫导致细胞增殖、ifn - γ、tnf - α和NO产生的反应下调（P < 0.01），但增加了tgf - β的释放（P < 0.001），而在这些动物中接种L3后则相反。在未免疫+L-3接种动物中，上述参数均下调（P < 0.01 ~ 0.001）。BmAFII免疫+ L-3攻击动物的细胞增殖反应Con A较大（P < 0.001），而BmAFII没有。免疫组、免疫+ L-3接种组和未免疫+L3接种组特异性IgG水平均高于未免疫组（P < 0.001），以免疫+ L-3接种组最高。总之，BmAFII免疫通过刺激对L-3的促炎反应来抑制L-3诱导的coucha感染的建立。(c) 2006 Elsevier Ltd.版权所有。
The present study was aimed at investigating protective efficacy of BmAFII (Sephadex G-200 eluted fraction of Brugia malayi adult worm extract) against establishment of infective larvae (L-3)-induced B. malayi infection in Mastomys coucha and to delineate immunological responses induced in the host. Healthy male M. coucha were immunized with BmAFII and subsequently inoculated with B. malayi L-3. Specific IgG and cell mediated immune responses (cellular proliferation) including release of tumor necrosis factor-alpha (TNF-alpha), interferon-gamma (IFN-gamma), transforming growth factor-beta (TGF-beta) and nitric oxide (NO) from host's cells stimulated with BmAFII or lipopolysaccharide (LPS)/concanavalin A (Con A) in vitro were determined. Immunization with BmAFII reduced the adult worm recovery by 85.7% (P < 0.001) and microfilaraemia by 77-95% of unimmunized controls (P < 0.05-0.01). Immunization alone resulted in downregulation of responses of cellular proliferation, IFN-gamma, TNF-alpha and NO production (P < 0.01) but increased TGF-beta release (P < 0.001) whereas the converse was seen after L3 inoculation in these animals. In unimmunized+L-3 inoculated animals all the above parameters were found downregulated (P < 0.01-0.001). The cell proliferative response of BmAFII immunized + L-3 challenged animals was larger for Con A (P < 0.001) but not for BmAFII. Specific IgG levels were higher in immunized, immunized + L-3 inoculated and unimmunized+L3 inoculated groups (P < 0.001) compared to unimmunized animals, the highest level being shown by immunized + L-3 inoculated group. In conclusion, immunization with BmAFII suppresses establishment of L-3-induced infection in M. coucha by stimulating proinflammatory responses to L-3. (c) 2006 Elsevier Ltd. All rights reserved.