Characterization of microRNA profile in IgE-mediated mouse BMMCs degranulation
Characterization of microRNA profile in IgE-mediated mouse BMMCs degranulation
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IgE 介导的小鼠 BMMC 脱颗粒中 microRNA 谱的表征
DOI：
10.1016/j.jmii.2018.10.006
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发表时间：
2020-08-01
影响因子：
7.4
通讯作者：
Wu，Zhengli
中科院分区：
文献类型：
--
作者：
Li，Yanhong;Liu，Jie;Wu，Zhengli
背景肥大细胞通过释放包括microRNA在内的预先形成和新合成的介质，在先天免疫和获得性免疫中发挥重要作用。虽然已经证实，在细胞增殖，分化，凋亡和免疫反应的功能，它们的功能仍然是有限的肥大细胞demetrating.MethodsHere，我们调查的miRNA表达谱在激活小鼠骨髓来源的肥大细胞（BMMCs）与miRNA微阵列和比较的配置文件，从休息BMMCs。选择部分miRNAs进行qPCR验证，选择let-7i进行肥大细胞脱颗粒过程中的功能研究。利用TargetScan Mouse数据库进行靶基因预测，基因本体（GO）进行基因分子功能分类，Cytoscape软件构建脱颗粒的基因网络。qPCR检测发现，在DNP刺激的骨髓基质细胞中，let-7 b、let-7 c、let-7 d、let-7 f、let-7i和miR-652表达上调，而miR-296- 3 p表达下调。在功能研究中，let-7i可通过抑制Exco 8表达而抑制肥大细胞脱颗粒。总之，数据表明miRNA参与肥大细胞活化，特别是肥大细胞脱粒过程。
BackgroundMast cells play a central role in innate and adaptive immunity by releasing pre-formed andde novosynthesized mediators, which include microRNAs. Although miRNAs have been confirmed to function in cell proliferation, differentiation, apoptosis, and the immune response, their functions are still limited in mast cells degranulation.MethodsHere, we survey miRNA expression profiles in activated mouse bone marrow-derived mast cells (BMMCs) with a miRNA microarray and compare the profiles to those from resting BMMCs. Partial miRNAs were selected for confirmation by qPCR, and let-7i was selected for function discover in mast cell degranulation process. TargetScan Mouse database were used for target genes prediction, gene ontology (GO) were used for gene molecular function classifications, and Cytoscape software were used to construct gene network of degranulation.ResultsWe found 13 up-regulated miRNAs and 7 down-regulated miRNAs in DNP activated BMMCs by miRNA microarray; and let-7b, let-7c, let-7d, let-7f, let-7i, and miR-652 were up-regulated, and miR-296-3p was down-regulated in DNP-stimulated BMMCs by qPCR. In the function research, let-7i can inhibit mast cell degranulation by suppress Exco8 expression. Overall, the data indicate that miRNAs participate in mast cell activation, especial for mast cell degranulation process.