Rheology of airway smooth muscle cells is associated with cytoskeletal contractile stress
Rheology of airway smooth muscle cells is associated with cytoskeletal contractile stress
复制标题
DOI：
10.1152/japplphysiol.00595.2003
复制
发表时间：
2004-05-01
影响因子：
3.3
通讯作者：
Fredberg, JJ
中科院分区：
文献类型：
--
作者：
Stamenovic, D;Suki, B;Fredberg, JJ
最近报道的培养气道平滑肌细胞的力学测量数据表明，细胞骨架基质的刚度取决于细胞骨架所承受的静态收缩应力的程度（Wang N, Tolic-Norrelykke IM, Chen J, Mijailovich SM, Butler JP, Fredberg JJ, and Stamenovic D. Am J Physiol Cell Physiol 282, C606 - C616, 2002）。另一方面，对这些细胞的流变学测量表明，细胞骨架刚度随施加机械载荷频率的变化呈幂律变化（Fabry B, Maksym GN, Butler JP, Glogauer M, Navajas DF, and Fredberg JJ）。物理通讯87:148102,2001)。在这项研究中，我们检查的可能性，这两个经验观察可能是相互关联的。我们将先前报道的人类气道平滑肌细胞收缩应力数据与描述这些细胞流变特性的新数据结合起来，得出了收缩应力与细胞流变学指标之间定量的、数学上可处理的、实验上可验证的经验关系。这些发现揭示了收缩应力的一个有趣的作用：尽管它在施加机械载荷下保持细胞的结构稳定性，但它也可能调节细胞骨架的流变特性，这对其他细胞功能至关重要。
Recently reported data from mechanical measurements of cultured airway smooth muscle cells show that stiffness of the cytoskeletal matrix is determined by the extent of static contractile stress borne by the cytoskeleton (Wang N, Tolic-Norrelykke IM, Chen J, Mijailovich SM, Butler JP, Fredberg JJ, and Stamenovic D. Am J Physiol Cell Physiol 282, C606 - C616, 2002). On the other hand, rheological measurements on these cells show that cytoskeletal stiffness changes with frequency of imposed mechanical loading according to a power law ( Fabry B, Maksym GN, Butler JP, Glogauer M, Navajas DF, and Fredberg JJ. Phys Rev Lett 87: 148102, 2001). In this study, we examine the possibility that these two empirical observations might be interrelated. We combine previously reported data for contractile stress of human airway smooth muscle cells with new data describing rheological properties of these cells and derive quantitative, mathematically tractable, and experimentally verifiable empirical relationships between contractile stress and indexes of cell rheology. These findings reveal an intriguing role of the contractile stress: although it maintains structural stability of the cell under applied mechanical loads, it may also regulate rheological properties of the cytoskeleton, which are essential for other cell functions.