Genetics of Candida albicans.
Genetics of Candida albicans.
复制标题
DOI：
10.1128/mmbr.54.3.226-241.1990
复制
发表时间：
1990-09
期刊：
影响因子：
--
通讯作者：
Stewart;'. Scherer;A. P. T. Magee
中科院分区：
文献类型：
--
作者：
Stewart;'. Scherer;A. P. T. Magee
白色念珠菌是最常见的真菌病原体之一。C.白色念珠菌和其他念珠菌属物种在免疫功能受损的个体中可能危及生命。对C.白色念珠菌的发病机制由于该物种的二倍体性质和缺乏已知的性周期而变得复杂。通过准有性技术和分子方法的结合，已经开发出有效的遗传系统。C.从白色念珠菌到研究更广泛的酿酒酵母，在念珠菌基因的分离和念珠菌的开发中具有很大的实用性。白念珠菌DNA转化系统。分子生物学方法已被用于阐明分类学关系和更精确的流行病学调查。分析了该菌的物理图谱和遗传图谱。白色念珠菌和密切相关的星状假丝酵母的研究提供了大量关于假丝酵母基因组高度流动性的信息。遗传系统在诸如耐药性、毒力决定因素和表型变异现象等生物学问题上的应用越来越多。虽然大多数的分子分析数据已经与C。白色念珠菌，相同的方法被证明对其他念珠菌属物种非常有效。
Candida albicans is among the most common fungal pathogens. Infections caused by C. albicans and other Candida species can be life threatening in individuals with impaired immune function. Genetic analysis of C. albicans pathogenesis is complicated by the diploid nature of the species and the absence of a known sexual cycle. Through a combination of parasexual techniques and molecular approaches, an effective genetic system has been developed. The close relationship of C. albicans to the more extensively studied Saccharomyces cerevisiae has been of great utility in the isolation of Candida genes and development of the C. albicans DNA transformation system. Molecular methods have been used for clarification of taxonomic relationships and more precise epidemiologic investigations. Analysis of the physical and genetic maps of C. albicans and the closely related Candida stellatoidea has provided much information on the highly fluid nature of the Candida genome. The genetic system is seeing increased application to biological questions such as drug resistance, virulence determinants, and the phenomenon of phenotypic variation. Although most molecular analysis to data has been with C. albicans, the same methodologies are proving highly effective with other Candida species.