METABOLIC SIGNALING BETWEEN PHOTORECEPTORS AND GLIAL-CELLS IN THE RETINA OF THE DRONE (APIS-MELLIFERA)
METABOLIC SIGNALING BETWEEN PHOTORECEPTORS AND GLIAL-CELLS IN THE RETINA OF THE DRONE (APIS-MELLIFERA)
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DOI：
10.1016/0006-8993(91)91432-z
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发表时间：
1991-12-13
期刊：
影响因子：
2.9
通讯作者：
TSACOPOULOS, M
中科院分区：
文献类型：
--
作者：
BRAZITIKOS, PD;TSACOPOULOS, M
实验证据表明代谢相互作用和信号之间的感光神经元和神经胶质细胞的蜜蜂雄蜂视网膜。在该组织中，[H-3]2-脱氧葡萄糖（[H-3]2DG）在黑暗中和重复光刺激期间几乎仅在神经胶质细胞中磷酸化为[H-3]2-脱氧葡萄糖-6P（[H-3]2DG-6P）。因此，刺激诱导的[H-3]2DG-6P形成速率的变化主要发生在神经胶质细胞中。用闪光重复刺激光感受器诱导胶质细胞中[H-3]2DG-6P的形成率增加约47%，这种效应可能是由于己糖激酶的激活。糖酵解的有效抑制剂碘乙酸（IAA）抑制了约75%的磷酸化。这可能主要是由于三磷酸腺苷（ATP）减少了约70%。将视网膜暴露于IAA抑制了光感受器中氧消耗（三角形QO 2）的瞬时上升，随后抑制了光诱导的感受器电位。这表明IAA大大减少了神经胶质细胞向光感受器提供糖酵解底物。缺氧，通过迅速抑制QO 2，取消了光感受器的受体电位，并导致视网膜ATP含量迅速下降约50%。同时，[H-3]2DG-6P的形成被抑制约30%。这表明呼吸光感受器向神经胶质细胞发送代谢信号，该信号被缺氧抑制。
Experimental evidence showing metabolic interaction and signaling between photoreceptors-neurons and glial cells of the honeybee drone retina is presented. In this tissue [H-3]2-deoxyglucose ([H-3]2DG) in the dark and during repetitive light stimulation is phosphorylated to [H-3]2-deoxyglucose-6P ([H-3]2DG-6P) almost exclusively in the glial cells. Hence, stimulus-induced changes in the rate of formation of [H-3]2DG-6P occurs predominantly in the glial cells. Repetitive stimulation of the photoreceptors with light flashes induced about a 47% rise in the rate of formation of [H-3]2DG-6P in the glial cells and this effect is probably due to the activation of hexokinase. The potent inhibitor of glycolysis iodoacetic acid (IAA), inhibited this phosphorylation by about 75%. Probably this was largely due to an about 70% decrease of adenosine triphosphate (ATP). Exposure of the retina to IAA suppressed the transient rise in oxygen consumption (triangle QO2) in the photoreceptors and subsequently the light-induced receptor potential. This indicates that the supply of a glycolytic substrate by glial cells to the photoreceptors is greatly reduced by IAA. Anoxia, by rapidly suppressing QO2, abolished the receptor potential of the photoreceptors and caused a rapid drop of about 50% in the ATP content of the retina. At the same time the formation of [H-3]2DG-6P was inhibited by about 30%. This indicates that respiring photoreceptors send a metabolic signal to glial cells which is suppressed by anoxia.