INTERLEUKIN-13 EXPRESSION IN THE NASAL-MUCOSA OF PERENNIAL ALLERGIC RHINITICS
INTERLEUKIN-13 EXPRESSION IN THE NASAL-MUCOSA OF PERENNIAL ALLERGIC RHINITICS
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DOI：
10.1164/ajrccm.152.6.8520776
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发表时间：
1995-12-01
影响因子：
24.7
通讯作者：
RA, CS
中科院分区：
文献类型：
--
作者：
PAWANKAR, RU;OKUDA, M;RA, CS
变应性鼻炎是IgE介导的特应性疾病，其特征在于在介导变应性反应中起核心作用的过敏原特异性IgE抗体水平升高。白细胞介素13（IL-13）是一种新的T细胞源性细胞因子，其与IL-4具有多种功能特性，并且已被证明能够诱导IgE合成。本研究旨在探讨IL-13基因在屋尘螨常年性变应性鼻炎（PAR）患者鼻粘膜上皮细胞中的表达，并与慢性感染性鼻炎（CIR）患者和正常人鼻粘膜上皮细胞进行比较。我们还研究了PAR患者外周血中IL-13基因的表达。分别采集甲状旁腺切除术患者和门诊患者的下鼻甲刮片，采用RT-PCR方法检测IL-13 mRNA的表达。采用密度梯度离心法分离外周血单个核细胞，RT-PCR法检测IL-13基因的表达。免疫组化法检测下鼻甲组织中IL-13蛋白表达及其细胞来源。分别采用PRIST法和CAP RAST法检测血清总IgE和过敏原特异性IgE抗体水平。IL-13基因在18/19例PAR患者鼻粘膜上皮中表达，但在正常志愿者和CIR患者中未检测到。PAR患者IL-13基因表达水平与血清总IgE（r = 0.80，p < 0.0001）和血清特异性IgE（r = 0.74，p < 0.0005）呈显著正相关。PAR患者外周血中未检测到IL-13基因表达。IL-13在蛋白水平的表达定位于淋巴细胞和肥大细胞。IL-13基因在PAR患者鼻粘膜上皮细胞中的选择性表达及其与血清IgE水平的强相关性提示其在PAR的发病机制中可能起重要作用，可能通过促进IgE的合成。PAR患者外周血中IL-13基因表达的缺失与其鼻粘膜上皮区室中的表达相反，表明IL-13可能在鼻粘膜微环境中局部发挥更重要的作用。
Allergic rhinitis is an IgE mediated atopic disease characterized by elevated levels of allergen specific IgE antibodies that play a central role in mediating allergic reactions. Interleukin 13 (IL-13) is a novel T-cell-derived cytokine that shares several functional properties with IL-4 and has been demonstrated to be capable of inducing IgE synthesis. The present study was designed to investigate the expression of IL-13 gene in the epithelial compartment of the nasal mucosa of patients with perennial allergic rhinitis (PAR) to house dust mite, comparing it with that in the nasal epithelial compartment of chronic infectious rhinitis (CIR) patients and normal volunteers (NV). We also investigated the IL-13 gene expression in the peripheral blood of PAR patients. Nasal scrapings were collected from the inferior turbinate of patients undergoing conchotomy surgery and from outpatients, and the mRNA expression of IL-13 was analyzed by the RT-PCR method. Peripheral blood mononuclear cells were isolated by density-gradient centrifugation and the expression of IL-13 gene was examined by the RT-PCR method. IL-13 expression at protein level and its cell source were analyzed by immunohistochemistry of inferior turbinate biopsies. The levels of total serum IgE and allergen-specific IgE antibodies in the serum were estimated by the PRIST and CAP RAST method, respectively. IL-13 gene expression was detected in the epithelial compartment of the nasal mucosa of 18/19 PAR patients but was undetected in normal volunteers and CIR patients. The level of IL-13 gene expression in PAR patients correlated strongly with the levels of total serum IgE (r = 0.80, p < 0.0001) and serum specific IgE (r = 0.74, p < 0.0005). Furthermore, IL-13 gene expression was not detected in the peripheral blood of PAR patients. IL-13 expression at protein level was localized to lymphocytes and mast cells. The selective expression of IL-13 gene in the epithelial compartment of the nasal mucosa of PAR patients and its strong correlation with the levels of serum IgE suggest its important role in the pathogenesis of PAR, probably by enhancement of IgE synthesis. The absence of IL-13 gene expression in the peripheral blood of PAR patients in contrast to its expression in the epithelial compartment of their nasal mucosa suggest that IL-13 may play a more important role locally within the microenvironment of the nasal mucosa.