Arabidopsis WRKY2 and WRKY34 transcription factors interact with VQ20 protein to modulate pollen development and function
Arabidopsis WRKY2 and WRKY34 transcription factors interact with VQ20 protein to modulate pollen development and function
复制标题
拟南芥WRKY2和WRKY34转录因子与VQ20蛋白相互作用调节花粉发育和功能
DOI：
10.1111/tpj.13619
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发表时间：
2017-09-01
期刊：
影响因子：
7.2
通讯作者：
Yu, Diqiu
中科院分区：
文献类型：
--
作者：
Lei, Rihua;Li, Xiaoli;Yu, Diqiu
植物雄配子发生受到严格的调控，涉及复杂而精确的转录重编程调控。WRKY转录因子在植物生长发育和逆境应答中起着重要作用。该家族的几个成员与含有VQ基序的蛋白（VQ蛋白）相互作用，以介导拟南芥中大量的程序;然而，WRKY-VQ复合物参与植物雄性配子发生仍然是未知的。在这项研究中，我们发现WRKY 2和WKRY 34在体外和体内都与VQ 20相互作用。进一步的实验表明，VQ 20的保守VQ基序负责它们的物理相互作用。VQ 20蛋白定位于细胞核，并在花粉中特异表达。表型分析表明，WRKY 2、WRKY 34和VQ 20对花粉发育和功能至关重要。WRKY 2、WRKY 34和VQ 20的突变同时导致雄性不育，花粉发育、萌发和花粉管生长缺陷。进一步的研究表明，VQ 20影响其相互作用的WRKY伙伴的转录功能。互补证据支持VQ 20的VQ基序对于花粉发育是必需的，因为VQ 20的突变形式（其中VQ基序中的LVQK残基被EDLE取代）不能拯救w2-1 w34-1 vq 20 -1三突变体植物的表型。进一步的表达分析表明，WRKY 2、WRKY 34和VQ 20共调控多个参与花粉发育、萌发和花粉管生长的基因。综上所述，我们的研究提供了证据表明，VQ 20作为一个关键的合作伙伴，WRKY 2和WKRY 34在植物雄性配子发生。
Plant male gametogenesis is tightly regulated, and involves complex and precise regulations of transcriptional reprogramming. WRKY transcription factors have been demonstrated to play critical roles in plant development and stress responses. Several members of this family physically interact with VQ motif-containing proteins (VQ proteins) to mediate a plethora of programs in Arabidopsis; however, the involvement of WRKY-VQ complexes in plant male gametogenesis remains largely unknown. In this study, we found that WRKY2 and WKRY34 interact with VQ20 both in vitro and in vivo. Further experiments displayed that the conserved VQ motif of VQ20 is responsible for their physical interactions. The VQ20 protein localizes in the nucleus and specifically expresses in pollens. Phenotypic analysis showed that WRKY2, WRKY34 and VQ20 are crucial for pollen development and function. Mutations of WRKY2, WRKY34 and VQ20 simultaneously resulted in male sterility, with defects in pollen development, germination and tube growth. Further investigation revealed that VQ20 affects the transcriptional functions of its interacting WRKY partners. Complementation evidence supported that the VQ motif of VQ20 is essential for pollen development, as a mutant form of VQ20 in which LVQK residues in the VQ motif were replaced by EDLE did not rescue the phenotype of the w2-1 w34-1 vq20-1 triple-mutant plants. Further expression analysis indicated that WRKY2, WRKY34 and VQ20 co-modulate multiple genes involved in pollen development, germination and tube growth. Taken together, our study provides evidence that VQ20 acts as a key partner of WRKY2 and WKRY34 in plant male gametogenesis.