Experimental Models in Syrian Golden Hamster Replicate Human Acute Pancreatitis.
Experimental Models in Syrian Golden Hamster Replicate Human Acute Pancreatitis.
复制标题
叙利亚金仓鼠复制人类急性胰腺炎的实验模型
DOI：
10.1038/srep28014
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发表时间：
2016-06-15
影响因子：
4.6
通讯作者：
Liu G
中科院分区：
文献类型：
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作者：
Wang Y;Kayoumu A;Lu G;Xu P;Qiu X;Chen L;Qi R;Huang S;Li W;Wang Y;Liu G
研究表明，仓鼠在新陈代谢方面与人类有很多相似之处。为了在仓鼠身上复制人类急性胰腺炎，我们比较研究了常用方法的疗效，如腹腔注射毛蛋白、l -精氨酸、逆行输注牛磺胆酸钠，以及另一种同时给药乙醇和脂肪酸的新模型。通过血清淀粉酶活性、病理评分、髓过氧化物酶活性和胰腺炎症因子的表达来评估胰腺炎的严重程度。结果支持病理性损伤的严重程度与采用相同方法诱导的小鼠和大鼠胰腺炎一致。其中，小蛋白诱导轻度水肿性胰腺炎伴轻微肺损伤，而l -精氨酸诱导极严重的胰腺损伤，包括坏死和中性粒细胞浸润。将na -牛磺胆酸钠注入胰管可引起胰腺头部坏死性胰腺炎和远端轻度炎症。乙醇与脂肪酸联合诱导急性胰腺炎的严重程度介于毛蛋白和l -精氨酸诱导程度之间，并伴有明显的炎症细胞浸润。鉴于脂质代谢特征的优势，仓鼠模型非常适合研究与人类代谢改变相关的胰腺炎。
The hamster has been shown to share a variety of metabolic similarities with humans. To replicate human acute pancreatitis with hamsters, we comparatively studied the efficacy of common methods, such as the peritoneal injections of caerulein, L-arginine, the retrograde infusion of sodium taurocholate, and another novel model with concomitant administration of ethanol and fatty acid. The severity of pancreatitis was evaluated by serum amylase activity, pathological scores, myeloperoxidase activity, and the expression of inflammation factors in pancreas. The results support that the severity of pathological injury is consistent with the pancreatitis induced in mice and rat using the same methods. Specifically, caerulein induced mild edematous pancreatitis accompanied by minimal lung injury, while L-arginine induced extremely severe pancreatic injury including necrosis and neutrophil infiltration. Infusion of Na-taurocholate into the pancreatic duct induced necrotizing pancreatitis in the head of pancreas and lighter inflammation in the distal region. The severity of acute pancreatitis induced by combination of ethanol and fatty acids was between the extent of caerulein and L-arginine induction, with obvious inflammatory cells infiltration. In view of the advantages in lipid metabolism features, hamster models are ideally suited for the studies of pancreatitis associated with altered metabolism in humans.