Synthesis, assembly, and turnover of alpha and beta-erythroid and nonerythroid spectrins in rat hippocampal neurons.
Synthesis, assembly, and turnover of alpha and beta-erythroid and nonerythroid spectrins in rat hippocampal neurons.
复制标题
大鼠海马神经元中α和β-红系血影蛋白和非红系血影蛋白的合成、组装和周转。
DOI：
10.1016/s0006-8993(99)02026-0
复制
发表时间：
1999
期刊：
影响因子：
2.9
通讯作者：
Goodman,SR
中科院分区：
文献类型：
--
作者：
Sangerman,J;Gard,AL;Shah,A;Goodman,SR
本文研究了培养的大鼠海马神经元α-红系、β-红系、α-非红系和β-非红系血影蛋白的合成和转运。[35S]蛋氨酸和亚基特异性抗体被用来标记和免疫沉淀新合成的血影蛋白，培养12-14天。在正常静息条件下进行的合成实验表明，新合成的α-红系/β-红系和α-非红系/β-非红系血影蛋白在可溶组分和不溶组分中的比例均为1/1。可溶和不溶的α和β红系血影蛋白转换迅速(半衰期为16-24分钟)。可溶性非红系α-血影蛋白(半衰期=80min)和β血影蛋白(半衰期=53min)的翻转速度慢于其不溶性(30-34min)。非红系α血影蛋白周转率与除非红系β血影蛋白外的其他测定结果有显著差异，表明这些亚基在组装到膜骨架之前受到保护，不会被快速蛋白质降解。
The synthesis and turnover of α-erythroid, β-erythroid, α-nonerythroid and β-nonerythroid spectrins was investigated in cultured rat hippocampal neurons. [35S ]methionine and subunit specific antibodies were used to label and immunoprecipitate newly synthesized spectrins in 12- to 14-day-old cultures. Synthesis experiments, performed under normal resting conditions, showed that the ratio of newly synthesized α-erythroid/β-erythroid and α-nonerythroid/β-nonerythroid spectrins is 1/1 (mol/mol) both in the soluble and insoluble fractions. Soluble and insoluble α and β erythroid spectrin turn over rapidly (half-life=16–24 min). Soluble nonerythroid α-spectrin (half-life=80 min) and β spectrin (half-life=53 min) turn over more slowly than their insoluble counterparts (30–34 min). The nonerythroid α spectrin turnover was significantly different (p<0.05) from the other measurements except for nonerythroid β spectrin, indicating that these subunits are protected from rapid proteolytic degradation until they are assembled in the membrane skeleton.