Expression of the Grape VaSTS19 Gene in Arabidopsis Improves Resistance to Powdery Mildew and Botrytis cinerea but Increases Susceptibility to Pseudomonas syringe pv Tomato DC3000.
Expression of the Grape VaSTS19 Gene in Arabidopsis Improves Resistance to Powdery Mildew and Botrytis cinerea but Increases Susceptibility to Pseudomonas syringe pv Tomato DC3000.
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葡萄 VaSTS19 基因在拟南芥中的表达提高了对白粉病和灰葡萄孢的抗性，但增加了对注射器假单胞菌番茄 DC3000 的敏感性
DOI：
10.3390/ijms18092000
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发表时间：
2017-09-17
影响因子：
5.6
通讯作者：
Wang X
中科院分区：
文献类型：
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作者：
Wang Y;Wang D;Wang F;Huang L;Tian X;van Nocker S;Gao H;Wang X
二苯乙烯合成酶（STS）是催化白藜芦醇化合物生物合成的关键酶，在抗病中发挥着重要作用。STS与病原体反应及其调控的分子途径尚不清楚。我们从中国野生葡萄葡萄属山葡萄中克隆了一个STS基因VaSTS 19。CV.“通化-3”，并将该基因转入拟南芥。然后，我们产生了表达VaSTS 19的拟南芥品系，并评估了VaSTS 19在各种病原体胁迫中的功能，包括白粉病、B。灰葡萄假单胞菌（Pseudomonasseringae pv.）番茄DC 3000（PstDC 3000）。VaSTS 19增强了对白粉病和B的抗性。cinerea，但增加了对PstDC 3000的敏感性。苯胺蓝染色显示，VaSTS 19转基因株系积累了更多的胼胝质相比，非转基因对照植物，并表现出较小的气孔孔径时，暴露于病原体相关的分子模式（鞭毛蛋白片段（flg 22）或脂多糖（LPS））。几个疾病相关基因的表达分析表明，VaSTS 19的表达增强防御反应，通过水杨酸（SA）和/或茉莉酸（JA）信号通路。这些发现为STS基因在防御病原体中的功能提供了更深入的了解，并更好地理解SA和JA途径之间的调控串扰。
Stilbene synthase (STS) is a key enzyme that catalyzes the biosynthesis of resveratrol compounds and plays an important role in disease resistance. The molecular pathways linking STS with pathogen responses and their regulation are not known. We isolated an STS gene, VaSTS19, from a Chinese wild grape, Vitis amurensis Rupr. cv. “Tonghua-3”, and transferred this gene to Arabidopsis. We then generated VaSTS19-expressing Arabidopsis lines and evaluated the functions of VaSTS19 in various pathogen stresses, including powdery mildew, B. cinerea and Pseudomonas syringae pv. tomato DC3000 (PstDC3000). VaSTS19 enhanced resistance to powdery mildew and B. cinerea, but increased susceptibility to PstDC3000. Aniline blue staining revealed that VaSTS19 transgenic lines accumulated more callose compared to nontransgenic control plants, and showed smaller stomatal apertures when exposed to pathogen-associated molecular patterns (flagellin fragment (flg22) or lipopolysaccharides (LPS)). Analysis of the expression of several disease-related genes suggested that VaSTS19 expression enhanced defense responses though salicylic acid (SA) and/or jasmonic acid (JA) signaling pathways. These findings provide a deeper insight into the function of STS genes in defense against pathogens, and a better understanding of the regulatory cross talk between SA and JA pathways.