Bovine Babesiosis Live Vaccine Production
Bovine Babesiosis Live Vaccine Production
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牛巴贝虫病活疫苗生产
DOI：
10.1196/annals.1373.059
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发表时间：
2006
期刊：
影响因子：
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通讯作者：
A. Alvarez
中科院分区：
文献类型：
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作者：
C. Rojas;J. V. Figueroa;A. Alvarado;Pedro Mejia;J. Mosqueda;A. Falcón;C. Vega;A. Alvarez
翻译后摘要：对牛血清和红细胞（RBC）的伽马辐照允许在体外培养的巴氏杆菌的增殖和生长， 并且具有潜在的应用以从基底上除去污染的病毒和细菌。用25 kGy ~（60）Co γ射线照射人工污染血清，能杀灭牛传染性气管炎（IBR）和牛病毒性腹泻（BVD）病毒，并能杀灭细菌。在体外培养条件下，用牛巴伯螨（B.牛），通过加入25 kGy的60 Co辐照血清，从24孔培养板繁殖到225 cm 2组织培养瓶中，获得2.4%至8.8%的寄生红细胞（PPE）百分比。用25 k戈伊照射的血清和10 ~ 70戈伊照射的红细胞，将适应Irrad S的感染红细胞转移到照射基质体外培养系统中。PPE范围为3.1至11。二联巴氏杆菌（B.用25 kGy的辐照S建立了二联体（bigemina），其在组织培养瓶中的增殖达到PPE 0.5 - 4.3，与未辐照的对照培养物（1.2-4.8）相比无统计学差异（P > 0.05）。B。通过加入经辐照的血清和RBC（分别为25 k戈伊和70戈伊），将双联感染的RBC转移到改良的培养系统中。在培养瓶中获得的PPE为0.8至4.2。结果表明，γ射线照射是一种消除牛血清中潜在病毒污染和消除细菌的合适方法，可以通过体外培养生产减毒活疫苗。
Abstract: Gamma irradiation on bovine serum and red blood cells (RBC) allows proliferation and growth of in vitro‐cultured Babesia sp., and has potential application to inactivate contaminating viruses and bacteria from the substrate. Gamma irradiation with 25 kGy in a source of 60Co was able to inactivate infectious bovine rinotracheitis (IBR) and bovine viral diarrhea (BVD) viruses in artificially contaminated serum; besides, bacteria were also eliminated. In vitro culture of Babesia bovis (B. bovis) in modified substrate, by adding irradiated serum with 60Co at 25 kGy was propagated from 24‐well culture plates to 225 cm2 tissue culture flasks, and percentages of parasitized erythrocytes (PPE) from 2.4% to 8.8% were obtained. Infected RBC adapted to Irrad S were transferred to the irradiated substrate in vitro culture system, by using serum irradiated at 25 kGy and RBC from 10 to 70 Gy. The PPE ranged from 3.1 to 11. Culture of Babesia bigemina (B. bigemina) was established with Irrad S (25 kGy); its propagation was achieved in tissue culture flasks reaching PPE from 0.5 to 4.3 with no statistical difference (P > 0.05) when compared to the nonirradiated control culture (1.2–4.8). B. bigemina‐infected RBCs were transferred to the modified culture system by adding irradiated serum and RBC (25 kGy and 70 Gy, respectively). PPE obtained in culture flasks were from 0.8 to 4.2. The results indicate that gamma irradiation is a suitable method to inactivate potential viral contamination and eliminate bacteria from bovine serum, to produce a live attenuated vaccine through the in vitro culture.