Highly sensitive fluorescence detection system for microfluidic lab-on-a-chip
Highly sensitive fluorescence detection system for microfluidic lab-on-a-chip
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DOI：
10.1039/c0lc00586j
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发表时间：
2011-01-01
期刊：
影响因子：
6.1
通讯作者：
Bradley, Donal D. C.
中科院分区：
文献类型：
--
作者：
Ryu, Gihan;Huang, Jingsong;Bradley, Donal D. C.
我们展示了一个紧凑，低成本和实用的荧光检测系统的芯片实验室的应用。该系统包括作为光源的市售InGaN发光二极管（501 nm）、有机或硅光电二极管检测器、吸收性染料涂覆的滤色器以及线性和反射偏振器。注射成型的聚苯乙烯微流控芯片被用作心脏标记物肌红蛋白和CK-MB的荧光免疫测定的平台。使用5.6 x 10（4）微珠μ l（-1）的TransFluoSphere（R）混悬液测量检测的光学限（LOD），其可等同于类似于3 nM荧光素当量浓度。对于肌红蛋白和CK-MB，人血浆免疫测定的LOD均为1.5 ng/ml。
We demonstrate a compact, low cost and practical fluorescence detection system for lab-on-a-chip applications. The system comprises a commercially available InGaN light emitting diode (501 nm) as light source, an organic or silicon photodiode detector, absorptive dye coated colour filters and linear and reflective polarisers. An injection moulded polystyrene microfluidic chip is used as the platform for fluorescence immunoassays for cardiac markers myoglobin and CK-MB. The optical limit of detection (LOD) is measured using a TransFluoSphere (R) suspension at 5.6 x 10(4) beads mu l(-1) which can be equated to similar to 3 nM fluorescein equivalent concentration. The LOD for the human plasma immunoassays is measured as 1.5 ng ml(-1) for both myoglobin and CK-MB.