Isolation and characterization of immortalized porcine aortic endothelial cell lines
Isolation and characterization of immortalized porcine aortic endothelial cell lines
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DOI：
10.1016/s0165-2427(02)00170-8
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发表时间：
2002-10-08
影响因子：
1.8
通讯作者：
Yélamos, J
中科院分区：
文献类型：
--
作者：
Carrillo, A;Chamorro, S;Yélamos, J
原代猪内皮细胞在培养中的寿命有限。经过四到五次传代后，它们趋于去分化并最终达到衰老。本研究的目的是建立永生化猪主动脉内皮细胞系（AOCs），以便于在体外研究涉及内皮的不同病理过程。用含有SV 40基因组的质粒转染原代猪主动脉内皮细胞（PAEC），并根据形态学和表型特征进行选择。流式细胞术分析表明，乙酰化低密度脂蛋白（Ac-LDL）的摄取和SLA I类，CD 29，CD 31，CD 41/61，CD 80/86，CD 46，SWC 3和LAMP-1抗原的组成型表达的所有分析的线，并显示出很小的差异，以原代细胞。原代和永生化内皮细胞之间的功能相似性在使用人自然杀伤细胞系（NKL）作为效应子的细胞毒性试验中得到证实。AOCs细胞系是体外研究人对猪内皮细胞免疫反应的重要工具。此外，它们将是非常有用的，以了解一些病毒性出血性疾病的猪，如非洲猪瘟（ASF）或经典猪瘟（CSF）的发病机制。(C)2002 Elsevier Science B. V.保留所有权利。
Primary porcine endothelial cells have a limited life span in culture. After four to five passages, they tend to de-differentiate and eventually reach senescence. The aim of this work was to establish immortalized porcine aortic endothelial cell lines (AOCs) to facilitate in vitro studies of different pathological process involving the endothelium. Primary porcine aortic endothelial cells (PAECs) were transfected with a plasmid containing the SV40 genome and selected on the basis of morphological and phenotypical features. Flow cytometry analysis demonstrated uptake of acetylated low density lipoproteins (Ac-LDL) and constitutive expression of SLA class I, CD29, CD31, CD41/61, CD80/86, CD46, SWC3, and LAMP-1 antigens by all analyzed lines and showed little differences to primary cells. The functional similarity between primary and immortalized endothelial cells was demonstrated in a cytotoxicity assay using a human natural killer cell line (NKL) as effector. The AOCs cell lines should be valuable tools for in vitro study of the human immune response against pig endothelial cells. In addition, they would be very useful to gain insight in the pathogenesis of some viral haemorrhagic diseases of pig such as African swine fever (ASF) or classical swine fever (CSF). (C) 2002 Elsevier Science B.V. All rights reserved.