Localization of monocyte chemoattractant peptide-1 expression in the central nervous system in experimental autoimmune encephalomyelitis and trauma in the rat.
Localization of monocyte chemoattractant peptide-1 expression in the central nervous system in experimental autoimmune encephalomyelitis and trauma in the rat.
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DOI：
10.4049/jimmunol.156.8.3017
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发表时间：
1996-04
影响因子：
4.4
通讯作者：
J. Berman;M. Guida;J. Warren;J. Amat;C. Brosnan
中科院分区：
文献类型：
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作者：
J. Berman;M. Guida;J. Warren;J. Amat;C. Brosnan
单核细胞趋化蛋白-1（MCP-1）是趋化因子β家族的成员，其已被证明在组织损伤部位的单核细胞和T细胞炎症的起始中起主要作用。在这项研究中，我们已经研究了MCP-1的表达分布在中枢神经系统（CNS）的炎症与自身免疫性疾病实验性自身免疫性脑脊髓炎（EAE），并与创伤相关的炎症检测结果进行了比较。在EAE中，在炎症发作时检测到MCP-1表达，在疾病的临床表达之前，在蛛网膜下腔位置的淋巴细胞和内皮细胞中。24 h后出现单核细胞浸润。在临床症状出现后，MCP-1表达广泛分布于脊髓中，其水平随着疾病活动而增加和减少。通过免疫反应和原位杂交，可以确定淋巴细胞、巨噬细胞、星形胶质细胞和内皮细胞为MCP-1的来源。巨噬细胞浸润和MCP-1的mRNA水平之间存在类似的密切相关性，发现在中枢神经系统的大鼠受到创伤，并在这些动物中，MCP-1的巨噬细胞和内皮细胞的免疫组化检测。结果支持MCP-1是CNS中炎症的重要介质的结论。
Monocyte chemoattractant protein-1 (MCP-1) is a member of the chemokine beta family of chemoattractants that has been shown to play a major role in the initiation of monocyte and T cell inflammation to sites of tissue injury. In this study, we have examined the distribution of MCP-1 expression in inflammation in the central nervous system (CNS) associated with the autoimmune disease experimental autoimmune encephalomyelitis (EAE) and compared the results with those detected in inflammation associated with trauma. In EAE, MCP-1 expression was detected at the onset of inflammation, prior to clinical expression of disease, in lymphocytes and endothelial cells in subarachnoid locations. Monocyte infiltration into these areas appeared 24 h later. After the onset of clinical signs, MCP-1 expression was widely distributed in the spinal cord with levels increasing and decreasing in association with disease activity. Lymphocytes, macrophages, astrocytes, and endothelial cells could be identified as sources of MCP-1 by immunoreactivity and in situ hybridization. A similar close correlation between macrophage infiltration and the levels of mRNA for MCP-1 was found in the CNS of rats subjected to trauma, and in these animals MCP-1 was detected by immunohistochemistry in macrophages and endothelial cells. The results support the conclusion that MCP-1 is an important mediator of inflammation in the CNS.