Cloning and functional expression of ATA1, a subtype of amino acid transporter A, from human placenta.
Cloning and functional expression of ATA1, a subtype of amino acid transporter A, from human placenta.
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来自人胎盘的氨基酸转运蛋白 A 的亚型 ATA1 的克隆和功能表达。
DOI：
10.1006/bbrc.2000.3061
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发表时间：
2000
期刊：
影响因子：
--
通讯作者：
Ganapathy,V
中科院分区：
文献类型：
--
作者：
Wang,H;Huang,W;Sugawara,M;Devoe,LD;Leibach,FH;Prasad,PD;Ganapathy,V
本报告描述了氨基酸转运系统A的一个亚型，人ATA 1的一级结构和功能特征。从胎盘cDNA文库中分离人ATA 1 cDNA。该cDNA编码487个氨基酸的蛋白质，具有11个推定的跨膜结构域。转运蛋白mRNA（约9.0 kb）在胎盘和心脏中表达最显著，但在其他组织（包括脑）中也有明显的可检测表达水平。当在哺乳动物细胞中异源表达时，克隆的转运蛋白介导系统A特异性模型底物α-（甲氨基）异丁酸的Na+偶联转运。转运过程是饱和的，米氏常数为0.89 ± 0.12 mM。根据Na+-活化动力学推导出的Na+：氨基酸化学计量比为1：1。该转运蛋白对小的短链中性氨基酸具有特异性。转运蛋白基因位于人类12号染色体上。
This report describes the primary structure and functional characteristics of human ATA1, a subtype of the amino acid transport system A. The human ATA1 cDNA was isolated from a placental cDNA library. The cDNA codes for a protein of 487 amino acids with 11 putative transmembrane domains. The transporter mRNA (∼9.0 kb) is expressed most prominently in the placenta and heart, but detectable level of expression is evident in other tissues including the brain. When expressed heterologously in mammalian cells, the cloned transporter mediates Na+-coupled transport of the system A-specific model substrate α-(methylamino)isobutyric acid. The transport process is saturable with a Michaelis-Menten constant of 0.89 ± 0.12 mM. The Na+:amino acid stoichiometry is 1:1 as deduced from the Na+-activation kinetics. The transporter is specific for small short-chain neutral amino acids. The gene for the transporter is located on human chromosome 12.