Association of LPHN3 rs6551665 A/G polymorphism with attention deficit and hyperactivity disorder in Korean children
Association of LPHN3 rs6551665 A/G polymorphism with attention deficit and hyperactivity disorder in Korean children
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DOI：
10.1016/j.gene.2015.04.033
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发表时间：
2015-07-15
期刊：
影响因子：
3.5
通讯作者：
Jin, Han Jun
中科院分区：
文献类型：
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作者：
Hwang, In Wook;Lim, Myung Ho;Jin, Han Jun
注意缺陷多动障碍(ADHD)是学龄儿童常见的高度遗传性疾病。其遗传率估计为80%-90%，但支撑这种疾病的遗传成分仍有待披露。最近有报道称LPHN3基因与ADHD之间存在高度一致的相关性。在本研究中，我们研究了韩国人LPHN3 rs6551665 A/G多态与ADHD的关系。研究中使用的样本包括150名ADHD儿童和322名对照组。ADHD儿童按DSM-IV诊断。采用Dupaul Parent ADHD评定量表评定ADHD症状。采用聚合酶链式反应-限制性片段长度多态性方法检测LPHN3 rs6551665单核苷酸多态。用卡方检验和精确检验检验病例组和对照组之间的Hardy-Weinberg平衡、基因型和等位基因频率差异以及优势比。LPHN3基因位点与Hardy-Weinberg的预期没有偏离。我们观察到ADHD儿童在基因型频率(p=0.01)和等位基因频率(p=0.02)方面与对照组显著相关。GG等位基因(OR 2.959，95%CI 1.416~6.184，P=0.003)和G等位基因过剩(OR 1.44，95%CI 1.062~1.945，P=0.02)。当仅对男性样本进行分析时，这种关联更明显(p=0.005)，男性对照和病例的OR为4.029(95%CI 1.597-10.164，p=0.002)，G等位基因与A等位基因的OR为1.46(95%C11.002-2.127，p=0.048)。因此，我们的结果表明，LPHN3 rs6551665的GG基因型和G等位基因可能对ADHD有显著影响，尽管需要更大的样本量和功能研究来进一步阐明这些发现。(C)2015爱思唯尔B.V.保留所有权利。
Attention deficit hyperactivity disorder (ADHD) is a common and highly heritable disorder of school-age children. Its heritability was estimated at 80-90% but the genetic component underpinning this disorder remains to be disclosed. Recently, a highly consistent association between latrophilin3 (LPHN3) gene and ADHD was reported. In the present study, we examined the association between the LPHN3 rs6551665 A/G polymorphism and ADHD in Korea. The samples used in the study consisted of 150 ADHD children and 322 controls. The ADHD children were diagnosed according to DSM-IV. ADHD symptoms were evaluated with Dupaul Parent ADHD Rating Scales. LPHN3 rs6551665 SNP was determined by PCR-RFLP. Hardy-Weinberg equilibrium, genotype and allele frequency differences between the case and the control, and odds ratio were examined using the chi-square and exact tests. The LPHN3 gene locus was found to have no deviation from the Hardy-Weinberg expectation. We observed a significant association between the ADHD children and control group in genotype frequency (p = 0.01) and allele frequency (p = 0.02). The ADHD children appeared to have a surplus of GG genotype (OR 2.959, 95% CI 1.416-6.184,p = 0.003) and G allele (OR 1.44,95% CI 1.062-1.945, p = 0.02). The association was more distinctive when analysis was confined to male samples (p = 0.005), the OR of male controls and cases was 4.029 (95% CI 1.597-10.164, p = 0.002) and the OR having G allele vs. A allele was 1.46 (95% C11.002-2.127, p = 0.048). Thus our results imply that the LPHN3 rs6551665 GG genotype and G allele may provide a significant effect on the ADHD, although larger sample sizes and functional studies are necessary to further elucidate these findings. (C) 2015 Elsevier B.V. All rights reserved.