Association of the C1155547T polymorphism in WNK4 gene with essential hypertension in Xinjiang Kazakhs
Association of the C1155547T polymorphism in WNK4 gene with essential hypertension in Xinjiang Kazakhs
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DOI：
10.3760/cma.j.issn.1003-9406.2010.05.015
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发表时间：
2010-10-10
期刊：
影响因子：
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通讯作者：
Jin Li
中科院分区：
文献类型：
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作者：
Cao Fei-fei;Zhang Hai-xia;Jin Li
目的探讨WNK赖氨酸缺陷蛋白激酶4(WNK4)基因C1155547T多态性是否与新疆哈萨克族人高血压(EH)相关，并探讨该基因多态性与环境因素交互作用对EH的影响。方法研究对象为556例高血压患者和341例正常血压对照。用Taqman探针实时定量聚合酶链式反应方法检测C1155547T。测定血糖、甘油三酯、总胆固醇等生化指标。所有结果均采用Logistic回归分析。采用加性模型分析WNK4基因C1155547T、多态与环境因素的交互作用对高血压的影响。结果病例组和对照组C1155547T基因多态性均符合Hardy-Weinberg平衡。等位基因频率差异有统计学意义(P=0.003)。患者组T等位基因频率显著高于对照组(P=0.002)。Logistic回归分析显示，年龄、体重指数(BMI)、总胆固醇以及CT+TT基因型频率是EH的危险因素。C1155547T基因多态与性别的正交互作用。观察体重指数、血糖的变化。OR值分别为3.85(95%CI：1.23~12.04)、5.91(95%CI：1.99~17.57)和8.77(95%CI：1.04~73.93)。结论WNK4基因第7外显子C1155547T多态可能与新疆哈萨克族人EH相关，T等位基因可能是高血压的危险因素。WNK4基因C1155547T多态与性别、体重指数、血糖存在交互作用。
Objective To investigate whether the WNK lysine deficient protein kinase 4 (WNK4) gene C1155547T polymorphism is associated with essential hypertension (EH) in Xinjiang Kazakhs and to assess the effect of the interaction between this polymorphism and environment factors on EH. Methods The study covered 556 hypertension patients and 341 normotensive controls. The C1155547T was determined by Taqman probe real-time PCR method. Some biochemical index such as glucose, triglyceride and total cholesterol were also measured. All of these results were analyzed with Logistic regression analysis. Additive model was applied to assess the effect of interaction between the WNK4 gene C1155547T, polymorphism and environment factors on hypertension. Results The C1155547T polymorphism was consistent with Hardy-Weinberg equilibrium in both the case and control groups. There was significant difference in the genotype frequencies (P=0.003). The T allele frequency was significantly higher in the patient group(P=0.002). Logistic regression analysis revealed that the age, body mass index (BMI), total cholesterol as well as the CT+TT genotype frequency conferred increased risks for EH. Positive interaction between the C1155547T polymorphism and gender. BMI, glucose was observed. The ORs were 3.85 (95% CI: 1.23-12.04), 5.91 (95% CI; 1.99-17.57) and 8.77 (95% CI; 1.04-73.93), respectively. Conclusion The result suggested that the exon 7 C1155547T polymorphism in WNK4 gene might be associated with EH in Xinjiang Kazakhs, the T allele might be the risk factor of essential hypertension. There were interactive effects between the WNK4 gene C1155547T polymorphism and gender, BMI and glucose.