Immunohistochemical localization of bone morphogenetic proteins (BMPs) 2, 4, 6, and 7 during induced heterotopic bone formation
Immunohistochemical localization of bone morphogenetic proteins (BMPs) 2, 4, 6, and 7 during induced heterotopic bone formation
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DOI：
10.1002/jor.20340
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发表时间：
2007-04-01
影响因子：
2.8
通讯作者：
Anderson, H. Clarke
中科院分区：
文献类型：
--
作者：
McCullough, Kirk A.;Waits, Chad A.;Anderson, H. Clarke
采用免疫组织化学方法检测Saos-2细胞源性植入物诱导Nu/Nu小鼠异位骨中骨形态发生蛋白（BMPs） 2、4、6、7的分布及染色强度。失活的Saos-2细胞或其提取物皮下植入Nu/Nu小鼠，可诱导软骨内成骨。BMP染色主要为细胞质染色。在肥大和凋亡的软骨细胞、骨膜和血管周围细胞等成骨细胞和成骨细胞中，BMP染色最强烈。骨细胞和破骨细胞的BMP染色是不同的，从无法检测到强烈染色，从诱导骨髓巨核细胞的少量到中度染色。Saos-2移植诱导骨的BMP-2、4、6和7染色表明：(1)Saos-2细胞产物促进宿主骨祖细胞表达bmp，进而导致异位部位的骨和骨髓形成；(2)成熟软骨细胞中可见强烈的BMP染色，因此可能在软骨细胞分化和/或凋亡中起作用；(3)血管周围细胞和骨膜细胞中BMP的表达表明成骨细胞也表达BMP；(4)破骨细胞释放BMP可促进骨重塑部位的成骨细胞分化。这些新数据有助于理解bmp在促进临床骨修复和各种病理条件下的作用。(c) 2007年骨科研究学会。Wiley期刊公司出版。
The distribution and staining intensity of bone morphogenetic proteins (BMPs) 2, 4, 6, and 7 were assessed by immunohistochemistry in ectopic bone induced in Nu/Nu mice by Saos-2 cell derived implants. Devitalized Saos-2 cells or their extracts can induce endochondral bone formation when implanted subcutaneously into Nu/Nu mice. BMP staining was mostly cytoplasmic. The most intense BMP staining was seen in hypertrophic and apoptotic chondrocytes, osteoprogenitor cells such as periosteal and perivascular cells, and osteoblasts. BMP staining in osteocytes and osteoclasts was variable, ranging from undetectable to intensely stained, and from minimal to moderately stained in megakaryocytes of the induced bone marrow. BMP-2, 4, 6, and 7 staining in Saos-2 implant-induced bone indicates the following: (1) Saos-2 cell products promote expression of BMPs by host osteoprogenitor cells, which in turn, leads to bone and marrow formation at ectopic sites; (2) strong BMP staining is seen in maturing chondrocytes, and thus may play a role in chondrocyte differentiation and/or apoptosis; (3) BMP expression in perivascular and periosteal cells indicates that osteoprogenitor cells also express BMP; (4) BMP release by osteoclasts may promote osteoblastic differentiation at sites of bone remodeling. These new data can be useful in understanding the role of BMPs in promoting clinical bone repair and in various pathologic conditions. (c) 2007 Orthopaedic Research Society. Published by Wiley Periodicals, Inc.