Detection of JC virus DNA in cerebrospinal fluid from multiple sclerosis patients
Detection of JC virus DNA in cerebrospinal fluid from multiple sclerosis patients
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DOI：
10.1177/135245859800400202
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发表时间：
1998-04-01
影响因子：
5.8
通讯作者：
Caputo, D
中科院分区：
文献类型：
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作者：
Ferrante, P;Omodeo-Zorini, E;Caputo, D
JC 病毒 (JCV) 是进行性多灶性白质脑病 (PML) 的病原体，已被认为是多发性硬化症 (MS) 的可能致病因素。我们进行了一项研究，通过巢式 PCR 在从 121 名 MS 患者、24 名其他神经系统疾病患者 (OND)、30 名非神经系统患者 (NND) 和 40 名健康受试者的 PBMC 和尿液中收集的脑脊液 (CSF)、外周血单核细胞 (PBMC) 和尿液样本中搜索 JCV 基因组的 LT 区域。在 11 名 MS 患者 (9%) 的脑脊液中发现了 JCV DNA，而 24 名 OND 和 30 名 NND 病例的所有脑脊液均为阴性。 MS 患者和对照组之间的 PBMC 和尿液中 JCV DNA 检测频率没有观察到显着差异。对 7 个 JCV CSF 分离株的核苷酸序列分析表明，其中 5 个菌株与原型菌株相同，而另外两个菌株在 4286 个核苷酸 (nt) 处存在碱基突变 (T-->C)。 MS 患者脑脊液中 JCV DNA 的发现表明 JCV 可能在 MS 致病过程的触发和/或维持中发挥作用，因此在监测该疾病时应予以考虑。
JC virus (JCV), the causative agent of progressive multifocal leukoencephalopathy (PML), has been proposed as a possible aetiopathogenic factor in multiple sclerosis (MS). We performed a study to search the LT region of JCV genome by nested PCR in cerebrospinal fluid (CSF), peripheral blood mononuclear cell (PBMC) and urine samples collected from 121 MS patients, 24 patients with other neurological disorders (OND), 30 non neurological patients (NND) and in PBMCs and urine of 40 healthy subjects. JCV DNA has been found in the CSF of 11 MS patients (9%) while all the CSFs from the 24 OND and the 30 NND cases were negative. No significant differences have been observed as regard to the frequency of JCV DNA detection in PBMCs and urine between the MS patients and the control groups. Nucleotide sequences analysis of seven JCV CSF isolates showed that five strains were identical the prototypal strain, while the other two had a base mutation (T-->C) in 4286 nucleotide (nt). The finding of JCV DNA in the CSF of MS patients suggest that JCV could ploy a role in the triggering and/or in the maintenance of MS aetiopathogenic process, and therefore it should be taken in consideration when monitoring this disease.