Monocotyledonous plants graft at the embryonic root–shoot interface
Monocotyledonous plants graft at the embryonic root–shoot interface
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DOI：
10.1038/s41586-021-04247-y
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发表时间：
2021-12
期刊：
影响因子：
64.8
通讯作者：
Gregory Reeves;Anoop Tripathi;Pankaj Singh;Maximillian R W Jones;A. K. Nanda;Constance Musseau;M. Craze;S. Bowden;Joseph F Walker;A. Bentley;Charles W. Melnyk;J. Hibberd
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文献类型：
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作者：
Gregory Reeves;Anoop Tripathi;Pankaj Singh;Maximillian R W Jones;A. K. Nanda;Constance Musseau;M. Craze;S. Bowden;Joseph F Walker;A. Bentley;Charles W. Melnyk;J. Hibberd
嫁接在动物和植物中都是可能的。虽然在动物中，这个过程需要外科手术，并且通常与排斥非自我有关，但在植物中，嫁接是普遍的，并且自古以来一直用于作物改良。然而，在单子叶植物中，它代表了第二大类陆生植物，包括许多主要作物，缺乏维管形成层被认为是排除嫁接。在这里，我们表明，胚胎下胚轴允许内和种间嫁接在所有三个单子叶植物组：鸭茅，百合和泽泻。我们通过组织学、外源荧光染料的应用、内源激素运动的互补测定以及植物生长至成熟来展示功能性嫁接联合。表达谱鉴定了统一与单子叶植物和双子叶植物中嫁接相关的分子反应的基因，以及以前与组织愈合无关的基因家族。小麦敏感接穗与燕麦砧木的融合赋予了对土传病原体小麦全蚀病菌的抗性。总的来说，这些数据推翻了单子叶植物不能形成嫁接联合的共识，并将下胚轴（禾本科植物中的中胚轴）确定为允许这一过程的分生组织。我们的结论是嫁接亲和性是一个共同的能力之间的种子轴承植物。
Grafting is possible in both animals and plants. Although in animals the process requires surgery and is often associated with rejection of non-self, in plants grafting is widespread, and has been used since antiquity for crop improvement. However, in the monocotyledons, which represent the second largest group of terrestrial plants and include many staple crops, the absence of vascular cambium is thought to preclude grafting. Here we show that the embryonic hypocotyl allows intra- and inter-specific grafting in all three monocotyledon groups: the commelinids, lilioids and alismatids. We show functional graft unions through histology, application of exogenous fluorescent dyes, complementation assays for movement of endogenous hormones, and growth of plants to maturity. Expression profiling identifies genes that unify the molecular response associated with grafting in monocotyledons and dicotyledons, but also gene families that have not previously been associated with tissue union. Fusion of susceptible wheat scions to oat rootstocks confers resistance to the soil-borne pathogenGaeumannomyces graminis. Collectively, these data overturn the consensus that monocotyledons cannot form graft unions, and identify the hypocotyl (mesocotyl in grasses) as a meristematic tissue that allows this process. We conclude that graft compatibility is a shared ability among seed-bearing plants.