Extracts from the roots of Lindera strychifolia induces apoptosis in lung cancer cells and prolongs survival of tumor-bearing mice
Extracts from the roots of Lindera strychifolia induces apoptosis in lung cancer cells and prolongs survival of tumor-bearing mice
复制标题
DOI：
10.1142/s0192415x03001545
复制
发表时间：
2003-01-01
影响因子：
5.7
通讯作者：
Fujiwara, H
中科院分区：
文献类型：
--
作者：
Li, YM;Ohno, Y;Fujiwara, H
番泻叶山胡椒（Lindera strychifolia）为樟科攀缘灌木，具有镇痛、止痉等作用。它还显示出对几种肿瘤细胞系的细胞毒性作用，并抑制大分子生物合成。本研究探讨了L。马钱子提取物对肺癌细胞的体外和体内模型。两种人肺癌细胞系A549（腺癌）和SBC-3（小细胞癌）以及非肿瘤细胞系3 T3-L1（小鼠成纤维细胞）经受L.马钱子提取物治疗。在肺癌细胞上，L.马钱子以剂量依赖性方式诱导细胞生长抑制。相反，浸提液对3 T3-L1细胞未显示任何显著的细胞毒性作用。因此，该提取物对肿瘤细胞具有特异性。L.马钱子提取物呈现典型的凋亡形态学特征，包括细胞核碎裂和细胞凝聚。L.在移植有刘易斯肺癌（LL-2）细胞的C57 BL/6小鼠和移植有A549或SBC-3人肺癌细胞的BALB/c裸鼠中研究了马钱子提取物。口服L.马钱子提取物通过诱导LL-2细胞模型中的细胞凋亡以剂量依赖的方式延长存活时间并抑制肿瘤生长。此外，在A549或SBC-3细胞裸鼠模型中，口服L.马钱子提取物在5.0mg/ml浓度下也显著抑制肿瘤生长。这些结果表明，L.马钱子具有抗癌活性，可能有助于临床应用于肺癌的预防和治疗。
Lindera strychifolia, a scandent shrub Lauraceous medicinal plant, has been used in Chinese traditional medicine as a palliative and an anti-spasmodic. It also shows cytotoxic effects against several tumor cell lines and inhibits marcromolecule biosynthesis. This study investigated the anti-tumor effects of L. strychifolia extract against lung cancer cells using in vitro and in vivo models. Two human lung cancer cell lines A549 (adenocarcinoma) and SBC-3 (small cell carcinoma), and a non-tumor cell line 3T3-L1 (mice fibroblasts) were subjected to L. strychifolia extract treatment. On lung cancer cells, L. strychifolia induced cell growth inhibition in a dose-dependent manner. Conversely, the extract did not show any ;significant cytotoxic effect on 3T3-L1 cells. Therefore, the extract is specific for tumor cells. Tumor cells treated with L. strychifolia extract showed typical morphological appearance of apoptosis including nuclei fragmentation and cell condensation. The in vivo effects of L. strychifolia extract were investigated in C57BL/6 mice transplanted with Lewis lung cancer (LL-2) cells, and in BALB/c nude mice transplanted with A549 or SBC-3 human lung cancer cells. Oral administration of L. strychifolia extract prolonged survival time and inhibited tumor growth in a dose-dependent manner by inducing apoptosis in the LL-2 cell mice model. Furthermore, in A549 or SBC-3 cell nude mice models, oral administration of L. strychifolia extract also significantly inhibited tumor growth at the 5.0 mg/ml concentration. These findings suggested that the components of L. strychifolia have anticancer activity and may contribute to clinical applications in the prevention and treatment of lung cancer.