LONG-TERM ANABOLIC EFFECTS OF PROSTAGLANDIN-E2 ON TIBIAL DIAPHYSEAL BONE IN MALE-RATS
LONG-TERM ANABOLIC EFFECTS OF PROSTAGLANDIN-E2 ON TIBIAL DIAPHYSEAL BONE IN MALE-RATS
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DOI：
10.1016/0169-6009(91)90109-d
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发表时间：
1991-10-01
期刊：
影响因子：
--
通讯作者：
LI, XJ
中科院分区：
文献类型：
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作者：
JEE, WSS;KE, HZ;LI, XJ
用7月龄雄性SD大鼠每日皮下注射前列腺素E_2(PGE_2)0、1、3和6 mg/kg/d，连续60、120和180天，观察长期前列腺素E_2(PGE_2)对胫骨骨干骨的影响。采用单光子骨密度仪对胫骨进行测量，对双荧光标记的未脱钙胫骨标本进行动态组织形态计量学分析。外源性前列腺素E_2在0~60天的时间内以剂量-反应方式产生以下瞬时变化：1)增加骨宽度和骨密度；2)增加总组织和总骨面积；3)减少骨髓面积；4)增加骨膜和皮质内膜板层骨形成；5)激活皮质内板层和编织小梁骨形成；6)激活皮质内骨重塑。从60d开始，观察到胫骨骨干骨量增加和骨活动增加的新的稳定状态。PGE2治疗60天后达到的骨量升高水平维持在120天和180天。这些观察表明，PGE2强大的合成代谢作用将增加骨膜和皮质内膜骨量，并维持每天连续服用PGE2的一过性骨量增加。
The effects of long-term prostaglandin E2 (PGE2) on tibial diaphyseal bone were studied in 7-month-old male Sprague-Dawley rats given daily subcutaneous injections of 0, 1, 3 and 6 mg PGE2/kg/day for 60, 120 and 180 days. The tibial shaft was measured by single photon absorptiometry and dynamic histomorphometric analyses were performed on double-fluorescent labeled undecalcified tibial diaphyseal bone samples. Exogenous PGE2 administration produced the following transient changes in a dose-response manner between zero and 60 days: 1) increased bone width and mineral density; 2) increased total tissue and total bone areas; 3) decreased marrow area; 4) increased periosteal and corticoendosteal lamellar bone formation; 5) activated corticoendosteal lamellar and woven trabecular bone formation and 6) activated intracortical bone remodeling. A new steady-state of increased tibial diaphyseal bone mass and elevated bone activities were observed from day 60 onward. The elevated bone mass level attained after 60 days of PGE2 treatment was maintained at 120 and 180 days. These observations indicate that the powerful anabolic effects of PGE2 will increase both periosteal and corticoendosteal bone mass and sustain the transient increase in bone mass with continuous daily administration of PGE2.