Exclusion of Ifa and Ifb as the Lps gene and mapping of three markers near the Lps locus.
Exclusion of Ifa and Ifb as the Lps gene and mapping of three markers near the Lps locus.
复制标题
排除 Ifa 和 Ifb 作为 Lps 基因，并对 Lps 基因座附近的三个标记进行定位。
DOI：
10.1007/s003359900569
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发表时间：
1997
期刊：
影响因子：
--
通讯作者：
Schwartz,DA
中科院分区：
文献类型：
--
作者：
Peiffer-Schneider,S;Schutte,BC;Murray,JC;Frees,KL;Williamson,K;Leysens,NJ;Schwartz,DA
通过直接测序研究了C3H/HeJ和C3H/OuJ品系小鼠的干扰素α和β基因（分别为Ifa和Ifbre）的单链构象多态性和直接测序。此外，在脂多糖低反应性小鼠与反应性 CastEi/C3H/HeJ 小鼠回交的后代中，还绘制了几个新的 4 号染色体标记，以及最近鉴定的位于干扰素 α 簇中的单链构象多态性。结果排除了脂多糖（Lps）基因的候选Ifa和Ifbas。鉴定出的 3 个新标记是 D4Mit322、D4Mit324 和 D4Mit 326。Lps 基因在遗传上与 D4Mit324 不可分离，但分别比 D4Mit322 和 D4Mit326 定位 1.2 和 2.5 cM。
The interferon α and β genes (IfaandIfbrespectively) in mice of the C3H/HeJ and C3H/OuJ strains were studied for single-strand conformation polymorphism and by direct sequencing. Also, several new chromosome 4 markers were mapped in progeny of a backcross of lipopolysaccharide hyporesponsive mice with responsive CastEi/C3H/HeJ mice, along with a recently identified single-strand conformation polymorphism located in the interferon α cluster. The results excludedIfaandIfbas candidates for the lipopolysaccharide (Lps) gene. The 3 new markers identified wereD4Mit322, D4Mit324andD4Mit 326. TheLpsgene was not genetically separable fromD4Mit324, but was mapped 1.2 and 2.5 cM fromD4Mit322andD4Mit326respectively.