Vasopressin- and neurophysin-immunoreactive neurons in the septal region, medial amygdala and locus coeruleus in colchicine-treated rats
Vasopressin- and neurophysin-immunoreactive neurons in the septal region, medial amygdala and locus coeruleus in colchicine-treated rats
复制标题
秋水仙碱治疗大鼠的间隔区、内侧杏仁核和蓝斑中的加压素和神经素免疫反应性神经元
DOI：
10.1016/0306-4522(85)90217-9
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发表时间：
1985
期刊：
影响因子：
3.3
通讯作者：
M. Sofroniew
中科院分区：
文献类型：
--
作者：
M. Sofroniew
用免疫组织化学方法观察了侧脑室注射秋水仙碱大鼠后叶加压素、催产素及其相关的神经垂体素神经元的分布和形态。在这些条件下，许多神经元含有加压素和neurophysin被发现在几个脑区除了那些先前描述的下丘脑。个别小细胞加压素神经元分散在内侧和外侧隔和垂直的分支的斜角带核，而大量的这样的神经元被发现整个床核的终纹和背侧部分的内侧杏仁核。此外，小细胞加压素神经元的集群是目前相邻的第三脑室的顶部在背侧下丘脑后区和一些这样的神经元被发现在腹侧蓝斑和蓝斑下。这些小细胞加压素神经元的平均直径为16.6 ~ 19.8 μm，而下丘脑大细胞加压素神经元的平均直径为25.4 μm，视交叉上核小细胞加压素神经元的平均直径为13.7 μm。在本研究中使用的条件下，在其他脑和脊髓区域中没有发现加压素神经元，尽管所有区域都进行了检查。在下丘脑尾侧室旁核内的催产素神经元的平均直径（18.9 μm）明显小于下丘脑其他部位的大细胞催产素神经元（24.8 μm）。这些具有实验记录的中枢连接的小细胞催产素神经元在大小和外观上与秋水仙碱处理后观察到的小细胞加压素神经元相似，后者是某些中枢加压素通路的潜在来源。这些发现表明，下丘脑中至少有两种类型的催产素神经元，大脑中各种不同区域中有几种类型的加压素神经元，其中许多都在下丘脑之外。
The distribution and morphology of neurons containing vasopressin, oxytocin and their associated neurophysins were examined immunohistochemically in rats given intracerebroventricular injections of colchicine. Under these conditions, numerous neurons containing vasopressin and neurophysin were found in several brain areas in addition to those previously described in the hypothalamus. Individual parvocellular vasopressin neurons were scattered in the medial and lateral septum and vertical limb of the nucleus of the diagonal band, while a large number of such neurons were found throughout both the bed nucleus of the stria terminals and the dorsal portion of the medial amygdala. In addition a small cluster of parvocellular vasopressin neurons was present adjacent to the top of the third ventricle in the posterior dorsal hypothalamic area and a number of such neurons were found in the ventral locus coeruleus and sub coeruleus. The mean diameters of these parvocellular vasopressin neurons ranged from 16.6 to 19.8 μm in the different regions, in contrast to the 25.4 μm mean diameter of hypothalamic magnocellular vasopressin neurons, or the 13.7 μm mean diameter of parvocellular vasopressin neurons in the suprachiasmatic nucleus. No vasopressin neurons were found in other brain and spinal cord regions under the conditions used in this study, although all regions were examined. No oxytocin neurons other than those previously described in the hypothalamus and immediately contiguous regions were found. Measurement of the mean diameter of oxytocin neurons showed that neurons in the caudal paraventricular nucleus were clearly smaller (18.9 μm) than magnocellular oxytocin neurons (24.8 μm) in other parts of the hypothalamus. These parvocellular oxytocin neurons with experimentally documented central connections were similar in both size and appearance to the parvocellular vasopressin neurons seen after colchicine treatment, which are potential sources of certain central vasopressin pathways.These findings indicate that there are at least two types of oxytocin neurons in the hypothalamus and several types of vasopressin neurons in a variety of different areas in the brain, many of which are outside of the hypothalamus.