レーザー誘起時間分解蛍光法を用いたin-vivo植物生葉のUV-B障害過程の検出
レーザー誘起時間分解蛍光法を用いたin-vivo植物生葉のUV-B障害過程の検出
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激光诱导时间分辨荧光法检测体内新鲜植物叶片UV-B损伤过程
DOI：
10.2184/lsj.30.666
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发表时间：
2002
期刊：
影响因子：
--
通讯作者：
彰夫 野村
中科院分区：
文献类型：
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作者：
竹内 麻希子;保典 斉藤;嘉明 中沢;琢也 川原;彰夫 野村
我们使用激光诱导荧光（LIF）方法来监测环境变化引起的植物损害。开发了一种时间分辨 LIF 测量系统，可监测快速荧光现象，采用 200 fs 和 660 nm 激光源以及时间分辨率为 30 ps 的光谱检测系统。制备了经 UV-B 照射的牵牛花的叶子样本，以研究系统监测损害过程的能力。在 685 nm 和 735 nm 处监测叶子的叶绿素荧光寿命。在这些波长下，LIF 寿命在 UV-B 照射期间变得更短，在停止照射后趋于恢复到控制值，然后最终再次表现出更短的寿命。讨论了寿命与植物活动之间的关系。
We used a laser-induced fluorescence (LIF) method to monitor plant damage caused by environmental changes. A time-resolved LIF measurement system which can monitor rapid fluorescence phenomena was developed with a 200-fs and 660-nm laser source and a spectroscopic detection system with a time resolution of 30 ps. Leaf samples of a morning glory irradiated by UV-B were prepared for investigating the capability of the system for monitoring the damage process. The chlorophyll fluorescence lifetime of the leaves was monitored at 685 nm and 735 nm. At those wavelengths, the LIF lifetimes became shorter during UV-B irradiation, tending to recover to the control values after quitting the irradiation, and then finally exhibiting shorter lifetimes again. Relationships between the lifetimes and the plant's activities are discussed.