Experimental SSM-CVB3 infection in macaques
Experimental SSM-CVB3 infection in macaques
复制标题
猕猴实验性 SSM-CVB3 感染
DOI：
10.1016/j.yexmp.2011.10.008
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发表时间：
2012-02-01
影响因子：
3.6
通讯作者：
Lin, Xijuan
中科院分区：
文献类型：
--
作者：
Han, Tiesuo;He, Wenqi;Lin, Xijuan
目的：方法：记录猕猴的临床症状，进行血液学、生化学和组织病理学检查，检测病毒滴度和中和抗体滴度。检测血清中SSM-CVB 3、柯萨奇病毒和腺病毒受体（CAR）和衰变加速因子（decay accelerating factor，ERF）的mRNA水平。SSM-CVB 3感染后，猕猴表现出缺乏活动，食欲不振，体温升高和严重腹泻。猕猴在接种后4至10天也出现血尿和蛋白尿。病毒滴度（5.1-6.5 LogICID（50）/mL）在接种后6 - 10天较高，NT滴度（6.5-7.3 Log 2）在接种后8 - 14天达到平台期。感染后猕猴出现严重贫血，红细胞、白细胞减少，淋巴细胞百分比升高。血清CK、CK-MB、AS和ALT分别为84-169 U/L、87.6-271.1 U/L、43-87 U/L和43-82 U/L，均高于正常值。组织学检查显示感染猕猴心、肝、肾损害明显，SSM-CVB 3 mRNA含量明显增加。感染猕猴的心、肝、肾组织中CAR和CRP均升高（P
Objective: To evaluate the pathogenicity of SSM-CVB3 in a macaque model.Methods: The clinical symptoms of macaques were recorded; hematological, biochemical and histopathological evaluations were completed; viral titers and neutralization titers (NT-titers) in sera were tested; and the mRNA levels of SSM-CVB3, coxsackievirus and adenovirus receptor (CAR) and decay accelerating factor (DAF) were determined.Results: After SSM-CVB3 infection, the macaques showed a lack of activity, a poor appetite, a higher body temperature, and severe diarrhea. The macaques also developed hematuria and albuminuria at 4 to 10 days post-inoculation. Virus titers (5.1-6.5 LogICID(50)/mL) were higher at 6 to 10 days post-inoculation, and NT-titers (6.5-7.3 Log2) reached plateaus at 8 to 14 days post-inoculation. The infected macaques developed serious anemia with decreased RBC and WBC, but the percentages of LYM were increased. The levels of CK, CK-MB, AS and ALT in the sera were 84-169 U/L, 87.6-271.1 U/L, 43-87 U/L and 43-82 U/L, respectively, and all of those were higher than normal. Histological analysis showed obvious cardiac, hepatic and renal damages in the infected macaques and the mRNA contents of SSM-CVB3. CAR and DAF in the heart, liver and kidneys of infected macaques were higher (P