Qualitative and quantitative determination of nucleosides, bases and their analogues in natural and cultured Cordyceps by pressurized liquid extraction and high performance liquid chromatography-electrospray ionization tandem mass spectrometry (HPLC-ESI-MS/MS)
Qualitative and quantitative determination of nucleosides, bases and their analogues in natural and cultured Cordyceps by pressurized liquid extraction and high performance liquid chromatography-electrospray ionization tandem mass spectrometry (HPLC-ESI-MS/MS)
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DOI：
10.1016/j.aca.2006.03.032
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发表时间：
2006-05-17
影响因子：
6.2
通讯作者：
Wang, Y. T.
中科院分区：
文献类型：
--
作者：
Fan, H.;Li, S. P.;Wang, Y. T.
建立了一种简便、快速的加压液体萃取（PLE）-高效液相色谱-电喷雾串联质谱（HPLC-ESI-MS/MS）联用技术，用于天然虫草和人工虫草中核苷、碱基及其类似物的定性和定量分析。使用PLE提取样品。采用ZORBAX Eclipse XDB-C-18色谱柱，以甲醇和5 mM乙酸铵水溶液为移动的相进行梯度洗脱，分离度为99.99%。通过表征其产物离子、前体离子和保留时间来鉴定目标化合物。采用时间程序选择性离子监测（SIM）或选择性反应监测（SRM），以正离子（尿苷为负）模式对研究化合物进行定量分析。结果表明，从冬虫夏草中检测到43种碱基、核苷及其类似物，并鉴定了其中16种化合物。建立了高效液相色谱-电喷雾串联质谱法同时测定冬虫夏草中7种核苷和6种碱基的方法。该方法线性好、选择性好、精密度高、回收率高、分析时间短，检出限和定量限均在ng/ml范围内。(c)2006 Elsevier B. V.保留所有权利。
A simple and rapid pressurized liquid extraction (PLE) and high performance liquid chromatography-electrospray ionization tandem mass spectrometry (HPLC-ESI-MS/MS) method was developed for qualitative and quantitative determination of nucleosides, bases and their analogues in natural and cultured Cordyceps. The samples were extracted using PLE. The separation was achieved on a ZORBAX Eclipse XDB-C-18 column with gradient elution of methanol and 5 mM aqueous ammonium acetate as mobile phase. Target compounds were identified by characterizing their product ions, precursor ions and retention times. Quantitative analysis of investigated compounds were performed using time programmed selective ion monitoring (SIM) or selective reaction monitoring (SRM) with 10 segments in positive (negative for uridine) ion mode. The results showed that 43 bases, nucleosides and their analogues were detected in Cordyceps, of these 16 compounds were identified. The simultaneous determination of seven nucleosides and six bases in Cordyceps was achieved using PLE and HPLC-ESI-MS/MS method described above, which afforded good linearity, selectivity, precision, recovery, short analysis time as well as LOD and LOQ in the ng/ml range. (c) 2006 Elsevier B.V. All rights reserved.