Halomarina oriensis gen. nov., sp. nov., a halophilic archaeon isolated from a seawater aquarium
Halomarina oriensis gen. nov., sp. nov., a halophilic archaeon isolated from a seawater aquarium
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DOI：
10.1099/ijs.0.020677-0
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发表时间：
2011-04-01
影响因子：
2.8
通讯作者：
Kogure, Kazuhiro
中科院分区：
文献类型：
--
作者：
Inoue, Kentaro;Itoh, Takashi;Kogure, Kazuhiro
从日本东京大学海洋研究所的海水水族馆中分离出一种新型嗜盐古菌，菌株KeC-11(T)。该菌株是需氧、革兰氏阴性和化学有机营养的，在 37 摄氏度、pH 7.0-8.0 和 2.7 M (16%) NaCl 中生长最佳。该菌株需要至少 10 mM 镁离子才能生长。 KeC-11(T)菌株的细胞是非运动的并且通常是不规则的球状或盘状。分离物的DNA G+C含量为67.7mol%。系统发育树重建表明它与盐杆菌科其他公认的成员关系较远，最近的亲缘关系是Natronomonas pharaonis Gabara(T)（序列相似性为91%）。该菌株含有磷脂酰甘油的C20C20和C20C25二醚衍生物、磷脂酰甘油磷酸甲酯、色谱上与糖基-甘露糖基-葡糖基二醚(TGD-2)相同的糖脂和至少一种未鉴定的糖脂。表型特征和系统发育数据支持将分离株KeC-11(T)置于盐杆菌科新属的新物种中，该属被命名为Halomarina oriensis gen。十一月，SP。十一月被提议；典型菌株是KeC-11(T) (=JCM 16495(T) =KCTC 4074(T))。
A novel halophilic archaeon, strain KeC-11(T), was isolated from a seawater aquarium at the Ocean Research Institute, University of Tokyo, Japan. The strain was aerobic, Gram-negative and chemo-organotrophic, growing optimally at 37 degrees C, at pH 7.0-8.0 and in 2.7 M (16%) NaCl. The strain required at least 10 mM magnesium ions for growth. Cells of strain KeC-11(T) were non-motile and generally irregular coccoids or discoids. The DNA G+C content of the isolate was 67.7 mol%. Phylogenetic tree reconstructions indicated that it was distantly related to the other recognized members of the family Halobacteriaceae, with the closest relative being Natronomonas pharaonis Gabara(T) (91% sequence similarity). The strain contained C20C20 and C20C25 diether derivatives of phosphatidylglycerol, phosphatidylglycerol phosphate methyl ester, a glycolipid chromatographically identical to the glycosyl-mannosyl-glucosyl diether (TGD-2) and at least one unidentified glycolipid. Phenotypic characterization and phylogenetic data support the placement of isolate KeC-11(T) in a novel species in a new genus within the family Halobacteriaceae, for which the name Halomarina oriensis gen. nov., sp. nov. is proposed; the type strain is KeC-11(T) (=JCM 16495(T) =KCTC 4074(T)).