The bonding property and cytotoxicity of a dental adhesive incorporating a new antibacterial monomer
The bonding property and cytotoxicity of a dental adhesive incorporating a new antibacterial monomer
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含有新型抗菌单体的牙科粘合剂的粘合性能和细胞毒性
DOI：
10.1111/j.1365-2842.2011.02212.x
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发表时间：
2011-11-01
影响因子：
2.9
通讯作者：
Chen, J.
中科院分区：
文献类型：
--
作者：
Chai, Z.;Li, F.;Chen, J.
已尝试引入可聚合阳离子单体来产生具有抗菌活性的牙科抗菌材料。本研究测试了将阳离子单体甲基丙烯酰氧基乙基十六烷基二甲基氯化铵（DMAE-CB）掺入商业牙科粘合剂中是否会影响母体粘合剂的粘合性能和生物相容性，以及DMAE-CB是否会从固化的改性粘合剂中浸出。比较DMAE-CB粘结剂与其亲本粘结剂与牙本质的微拉伸粘结强度。将经改性粘结剂粘结的牙本质盘在三种不同温度的人工唾液中孵育90天，以获得洗脱液。采用3-（4，5-二甲基噻唑-2-基）-2，5-二苯基溴化四氮唑（MTT）裂解法检测DMAE-CB单体和粘附洗脱液的细胞毒性。用改性胶粘剂的洗脱液进行高效液相色谱（HPLC）。结果表明，DMAE-CB的加入对牙本质粘接强度无明显影响（P > 0.05）。当DMAE-CB单体浓度为2 μ g/mL（-1）或更高时，对L929细胞有毒性作用（P < 0.05），而加入DMAE-CB的粘合剂洗脱液对细胞生长无明显影响（P > 0.05）。此外，HPLC分析检测到改性粘合剂的洗脱液的四种物质的峰基线分离，这与母体粘合剂的洗脱液相同，表明即使在浸泡90天后也没有可检测到的DMAE-CB单体浸出。这些结果表明，DMAE-CB可以稳定地掺入牙科粘合剂中，而不影响其母体粘合剂的粘合效率和生物相容性。
The incorporation of polymerisable cationic monomers has been attempted to generate dental resinous materials with antibacterial activity. This study tested whether the incorporation of a cationic monomer, methacryloxylethyl cetyl dimethyl ammonium chloride (DMAE-CB), into a commercial dental adhesive would influence the bonding property and biocompatibility of the parental adhesive, and whether DMAE-CB could leach out from the cured modified adhesive. Microtensile bond strengths of DMAE-CB-incorporated adhesive and its parental adhesive to dentin were compared. Dentin discs bonded with modified adhesive were incubated in artificial saliva at three different temperatures for 90 days to obtain eluents. The cytotoxicity of DMAE-CB monomer and adhesive eluents were tested with 3-(4, 5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide cleavage assay (MTT assay). High-performance liquid chromatography (HPLC) was performed with the eluents of the modified adhesive. The results indicated that the incorporation of DMAE-CB into the dental adhesive did not adversely influence its bonding strength to dentin (P > 0.05). Although DMAE-CB monomer exhibited toxicity against L929 cells at the concentration of 2 mu g mL(-1) or higher (P < 0.05), the eluents of DMAE-CB-incorporated adhesive did not show significant influence on cell growth (P > 0.05). Moreover, HPLC analysis detected four substances' peak baseline separation for the eluents of modified adhesives, which was identical to the eluent of the parental adhesive, indicating no detectable DMAE-CB monomer leaching even after soaking for 90 days. Those results suggest that DMAE-CB could be incorporated into the dental adhesive stably without compromising the bonding efficiency and biocompatibility of its parental adhesive.