Effect of total flavonoids of Spatholobus suberectus Dunn on PCV2 induced oxidative stress in RAW264.7 cells.
Effect of total flavonoids of Spatholobus suberectus Dunn on PCV2 induced oxidative stress in RAW264.7 cells.
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DOI：
10.1186/s12906-017-1764-6
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发表时间：
2017-05-02
影响因子：
--
通讯作者：
Hu TJ
中科院分区：
文献类型：
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作者：
Chen HL;Yang J;Fu YF;Meng XN;Zhao WD;Hu TJ
研究鸡血藤总黄酮（TFSD）对猪圆环病毒2（PCV 2）诱导的RAW 264. 7细胞氧化应激的影响。用PCV 2感染RAW 264. 7细胞建立氧化应激模型。然后用各种浓度（25 mg/ml、50 mg/ml和100 mg/ml）的TFSD处理病毒感染的细胞。采用紫外分光光度法、荧光法和商品化检测试剂盒，检测大鼠血清中氧化应激相关分子（NO、ROS、GSH和GSSG）水平及相关酶（SOD、MPO和XOD）活性。PCV 2感染后，RAW264.7细胞NO分泌、ROS产生、GSSG含量、XOD和MPO活性显著增加，GSH含量和SOD活性显著降低（P < 0.05）。经TFSD处理后，PCV 2引起的氧化应激相关分子水平和酶活性的变化恢复到与对照相似的水平。我们的研究结果表明，TFSD能够调节RAW264.7细胞中PCV 2感染诱导的氧化应激，这支持了该草药作为活性氧相关病理学的替代或补充治疗药物的民族医学用途。
This study was carried out to investigate the effect of total flavonoids of Spatholobus suberectus Dunn (TFSD) on PCV2 induced oxidative stress in RAW264.7 cells. Oxidative stress model was established in RAW264.7 cells by infecting with PCV2. Virus infected cells were then treated with various concentrations (25 mg/ml, 50 mg/ml and 100 mg/ml) of TFSD. The levels of oxidative stress related molecules (NO, ROS, GSH and GSSG) and activities of associated enzymes (SOD, MPO and XOD were analyzed using ultraviolet spectrophotometry, fluorescence method and commercialized detection kits. PCV2 infection induced significant increase of NO secretion, ROS generation, GSSG content, activities of both XOD and MPO, and dramatically decrease of GSH content and SOD activity in RAW264.7 cells (P < 0.05). After treating with TFSD, PCV2 induced alteration of oxidative stress related molecule levels and enzyme activities were recovered to a level similar to control. Our findings indicated that TFSD was able to regulate oxidative stress induced by PCV2 infection in RAW264.7 cells, which supports the ethnomedicinal use of this herb as an alternative or complementary therapeutic drug for reactive oxygen-associated pathologies.