Expression of Autotaxin, an Ectoenzyme that Produces Lysophosphatidic Acid, in Human Placenta
Expression of Autotaxin, an Ectoenzyme that Produces Lysophosphatidic Acid, in Human Placenta
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DOI：
10.1111/j.1600-0897.2009.00715.x
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发表时间：
2009-08-01
影响因子：
3.6
通讯作者：
Taketani, Yuji
中科院分区：
文献类型：
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作者：
Iwasawa, Yuki;Fujii, Tomoyuki;Taketani, Yuji
溶血磷脂酸（LPA）是一种生物活性脂质介质，被认为在妊娠中起重要作用。血浆LPA由自分泌运动因子（ATX）产生，妊娠期间血浆中ATX活性随孕周增加而增加，分娩后不久降至接近非妊娠水平。然而，妊娠期间ATX增加的来源仍然不确定。我们推测，增加ATX的来源可能是胎盘。方法的研究我们调查了ATX的蛋白和mRNA的表达，在人类胎盘使用免疫组化和RT-PCR，respectively.ResultsAt所有3妊娠trimesters，胎盘组织的免疫组化染色揭示了最显着的阳性染色的ATX蛋白在滋养层细胞。实时荧光定量PCR显示胎盘组织中ATX mRNA的表达量随孕周的增加而增加，即血浆中ATX水平随孕周的增加而增加。
ProblemLysophosphatidic acid (LPA) is a bioactive lipid mediator and thought to play an important role in pregnancy. Plasma LPA is produced by autotaxin (ATX), and ATX activity in plasma increases during pregnancy paralleled with gestational weeks and decreases to near the non-pregnant level soon after delivery. However, the source of increased ATX during pregnancy is still uncertain. We hypothesized that the source of increased ATX might be placenta.Method of studyWe investigated the protein and mRNA expression of ATX in human placenta using immunohistochemistry and RT-PCR, respectively.ResultsAt all 3 gestational trimesters, immunohistochemical staining for placenta tissues revealed the most marked positive staining of ATX protein in trophoblasts. Real-time PCR revealed that mRNA amounts of ATX in placenta tissues paralleled with gestational weeks, i.e. ATX level in plasma.ConclusionThese findings suggest that trophoblasts might produce ATX and its bioactive resultant substance, LPA, paralleled with gestational weeks.