Modeling Microtubule-Cytoplasm Interaction in Plant Cells
Modeling Microtubule-Cytoplasm Interaction in Plant Cells
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DOI：
10.1101/2022.09.29.510138
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发表时间：
2022-09
期刊：
影响因子：
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通讯作者：
Mohammad N. Murshed;Donghui Wei;Ying Gu;Jin Wang
中科院分区：
文献类型：
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作者：
Mohammad N. Murshed;Donghui Wei;Ying Gu;Jin Wang
虽然植物细胞中的微管已被广泛研究，但调控微管空间组织的机制尚不清楚。我们假设微管与细胞质流动之间的相互作用在微管的组装和定向中起着重要作用。为了验证这一假设，我们基于流固相互作用的理论和方法开发了一种新的微管计算建模框架。我们采用浸入边界法来跟踪微管在细胞质流动中的运动。我们还结合了两个微管相互碰撞时相遇动力学的细节。我们通过几个数值测试验证了我们的计算模型，然后将其应用于植物细胞生长中的微管-细胞质相互作用的模拟。我们的计算研究表明，微管主要在与细胞伸长轴正交的方向上定向。通过与实验室测量结果的对比，验证了仿真结果。我们发现，通过进一步的校准，我们的计算模型能够产生与实验结果在定性和定量上一致的微管取向模式。本研究提出的计算模型可以自然地扩展到许多涉及微结构与细胞内液相互作用的其他细胞系统。
Although microtubules in plant cells have been extensively studied, the mechanisms that regulate the spatial organization of microtubules are poorly understood. We hypothesize that the interaction between microtubules and cytoplasmic flow plays an important role in the assembly and orientation of microtubules. To test this hypothesis, we developed a new computational modeling framework for microtubules based on theory and methods from the fluid-structure interaction. We employed the immersed boundary method to track the movement of microtubules in cytoplasmic flow. We also incorporated details of the encounter dynamics when two microtubules collide with each other. We verified our computational model through several numerical tests before applying it to the simulation of the microtubule-cytoplasm interaction in a growing plant cell. Our computational investigation demonstrated that microtubules are primarily oriented in the direction orthogonal to the axis of cell elongation. We validated the simulation results through a comparison with the measurement from laboratory experiments. We found that our computational model, with further calibration, was capable of generating microtubule orientation patterns that were qualitatively and quantitatively consistent with the experimental results. The computational model proposed in this study can be naturally extended to many other cellular systems that involve the interaction between microstructures and the intracellular fluid.