Toxin profiles of five geographical isolates of Dinophysis spp. from North and South America
Toxin profiles of five geographical isolates of Dinophysis spp. from North and South America
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DOI：
10.1016/j.toxicon.2010.12.002
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发表时间：
2011-02-01
期刊：
影响因子：
2.8
通讯作者：
Anderson, Donald M.
中科院分区：
文献类型：
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作者：
Fux, Elie;Smith, Juliette L.;Anderson, Donald M.
海生甲藻可产生冈田酸(OA)和果胶毒素(PTX)类毒素。这些亲脂性毒素在滤食性贝类体内积累，并在消费者中引起一种称为腹泻性贝类中毒(DSP)的疾病。2008年，Dinophysis的一次水华导致德克萨斯州海岸的贝类捕捞区关闭，这是美国首批与DSP相关的关闭区域之一。这一事件导致对Dinophysis spp分离株的毒素产生进行了广泛的研究。来自美国沃特斯。在本研究中，我们比较了在实验室相同条件下，从美国(鳗鱼池塘、马萨诸塞州伍兹霍尔、马萨诸塞州玛莎葡萄园岛和得克萨斯州阿兰萨斯湾港)收集的Dinophysis地理分离物，以及来自加拿大(布莱克港、芬迪湾)和智利(Reloncai Estuary)的Dinophysis地理分离物的毒素谱。对每个分离株的线粒体cox1基因进行了测序，以帮助进行物种鉴定。来自美国东北部和加拿大的菌株都被归入Dinophysis acumata，而来自智利和德克萨斯州的菌株最有可能属于D.acumata复合体，而这个标记不能做出准确的物种划分。在所有Dinophysis分离物中都检测到毒素，并且每个分离物都有不同的特征。鳗鱼池塘、玛莎葡萄园岛和芬迪湾的分离物的毒素谱最相似，都含有OA。DTX1和PTX2。鳗鱼塘分离株还含有OA-D8和DTX1-D7，以及低水平(结构上未证实)的DTX1-D8和DTX1-D9。来自玛莎葡萄园的尖头盘藻产生DTX1-D7，以及在细胞和培养基中鉴定的OA、DTX1和PTX2。来自芬迪湾的尖吻假单胞菌产生DTX1和PTX2，在细胞和培养上清液中都可以检测到，而在培养上清液中只检测到微量的OA。来自德克萨斯州的Dinophysis菌株只产生OA，而来自智利的菌株只产生PTX2，这在细胞和培养基中都得到了证实。爱思唯尔有限公司出版。
Marine dinoflagellates of the genus Dinophysis can produce toxins of the okadaic acid (OA) and pectenotoxin (PTX) groups. These lipophilic toxins accumulate in filter-feeding shellfish and cause an illness in consumers called diarrhetic shellfish poisoning (DSP). In 2008, a bloom of Dinophysis led to the closure of shellfish harvesting areas along the Texas coast, one of the first DSP-related closures in the U.S. This event resulted in a broad study of toxin production in isolates of Dinophysis spp. from U.S. waters. In the present study, we compared toxin profiles in geographical isolates of Dinophysis collected in the U.S. (Eel Pond, Woods Hole MA; Martha's Vineyard, MA; and Port Aransas Bay, Texas), and in those from Canada (Blacks Harbour, Bay of Fundy) and Chile (Reloncavi Estuary), when cultured in the laboratory under the same conditions. For each isolate, the mitochondrial cox1 gene was sequenced to assist in species identification. Strains from the northeastern U.S. and Canada were all assigned to Dinophysis acuminata, while those from Chile and Texas were most likely within the D. acuminata complex whereas precise species designation could not be made with this marker. Toxins were detected in all Dinophysis isolates and each isolate had a different profile. Toxin profiles of isolates from Eel Pond, Martha's Vineyard, and Bay of Fundy were most similar, in that they all contained OA. DTX1, and PTX2. The Eel Pond isolate also contained OA-D8 and DTX1-D7, and low levels (unconfirmed structurally) of DTX1-D8 and DTX1-D9. D. acuminata from Martha's Vineyard produced DTX1-D7, along with OA, DTX1, and PTX2, as identified in both the cells and the culture medium. D. acuminata from the Bay of Fundy produced DTX1 and PTX2, as found in both cells and culture medium, while only trace amounts of OA were detected in the medium. The Dinophysis strain from Texas only produced OA, and the one from Chile only PTX2, as confirmed in both cells and culture medium. Published by Elsevier Ltd.