Influence of ibuprofen on phospholipid membranes
Influence of ibuprofen on phospholipid membranes
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DOI：
10.1103/physreve.91.022716
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发表时间：
2015-02-24
影响因子：
2.4
通讯作者：
Nickel, Bert
中科院分区：
文献类型：
--
作者：
Jaksch, Sebastian;Lipfert, Frederik;Nickel, Bert
对生物膜的基本了解至关重要，因为这些膜构成了生命本身的基石。细胞的功能取决于膜的一系列性质，这些性质对于细胞的稳定性和功能、信息和营养物质的运输、废物的处理以及最终允许药物进入细胞以及细菌和病毒的偏转都是重要的。我们已经调查了布洛芬的结构和动力学的L-α-磷脂酰胆碱（CIPPC）膜的掠入射小角中子散射，中子反射仪，掠入射中子自旋回波光谱的影响。从这些实验的结果，我们能够确定，布洛芬诱导的两个步骤的结构化行为在CNOPC膜，其中的结构演变从纯的层状相的纯CNOPC在两个六方相的叠加在高浓度的纯六方相。当膜中不存在布洛芬时可见的松弛在加入布洛芬后消失。我们把这归因于膜的硬化。这种行为可能有助于解释布洛芬在长期应用中的毒性行为。
A basic understanding of biological membranes is of paramount importance as these membranes comprise the very building blocks of life itself. Cells depend in their function on a range of properties of the membrane, which are important for the stability and function of the cell, information and nutrient transport, waste disposal, and finally the admission of drugs into the cell and also the deflection of bacteria and viruses. We have investigated the influence of ibuprofen on the structure and dynamics of L-alpha-phosphatidylcholine (SoyPC) membranes by means of grazing incidence small-angle neutron scattering, neutron reflectometry, and grazing incidence neutron spin echo spectroscopy. From the results of these experiments, we were able to determine that ibuprofen induces a two-step structuring behavior in the SoyPC films, where the structure evolves from the purely lamellar phase for pure SoyPC over a superposition of two hexagonal phases to a purely hexagonal phase at high concentrations. A relaxation, which is visible when no ibuprofen is present in the membrane, vanishes upon addition of ibuprofen. This we attribute to a stiffening of the membrane. This behavior may be instrumental in explaining the toxic behavior of ibuprofen in long-term application.