Experimental studies on comparison of the vector competence of four Italian Culex pipiens populations for West Nile virus.
Experimental studies on comparison of the vector competence of four Italian Culex pipiens populations for West Nile virus.
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DOI：
10.1186/s13071-015-1067-z
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发表时间：
2015-09-17
影响因子：
3.2
通讯作者：
Ciufolini MG
中科院分区：
文献类型：
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作者：
Fortuna C;Remoli ME;Di Luca M;Severini F;Toma L;Benedetti E;Bucci P;Montarsi F;Minelli G;Boccolini D;Romi R;Ciufolini MG
西尼罗河病毒(WNV)是一种媒介传播的疾病，导致世界许多地区的流行病。该病毒在自然界中由地方性鸟类-蚊子-鸟类循环维持，偶尔会传播给其他宿主，如马和人类。库蚊，特别是无处不在的淡色库蚊，被认为在西尼罗河病毒的地方性和流行性的维持和传播中发挥着重要的媒介作用。自2008年在欧洲引入以来，西尼罗河病毒主要在意大利东北部地区稳定传播，尽管有零星的马和/或人病例，以及西尼罗河病毒感染Cx。在意大利的其他地区也有记录。我们的研究范围是评估一些意大利CX种群的潜在能力。评估蚊子传播西尼罗河病毒的能力，并评估其垂直传播病毒的能力。为此，来自不同地区的四个意大利人被实验感染。在感染血餐后，对喂养的雌性动物进行为期32天的监测，以确定病毒的外在潜伏期。用实时定量聚合酶链式反应和Vero细胞空斑形成单位(pfu/m L)滴定方法检测感染蚊体的西尼罗河病毒滴度。通过检测喂食雌鼠的身体、腿和翅膀和唾液中的病毒，评估潜在的感染率、传播率和传播率(IR、DR、TR)。所有受试人群都对西尼罗河病毒感染易感。西尼罗河病毒在腿和翅膀上的存在表明了西尼罗河病毒在蚊子中传播的能力。在受试人群的唾液中检测到病毒RNA。在四个被研究的群体中，TR值没有显著差异。CX的后代。感染蚊虫的女性均为西尼罗河病毒阴性。我们的研究解决了意大利西尼罗河病毒与媒介的复杂关系知识中的一个重要问题，表明所有意大利Cx。被测种群对西尼罗河病毒具有媒介能力。应该进行进一步的研究，以便更好地阐明其他因素(媒介密度、气候条件、水库存在等)的作用。以便预测西尼罗河病毒可能在何时何地爆发。
West Nile virus (WNV) is a vector-borne disease responsible for causing epidemics in many areas of the world. The virus is maintained in nature by an enzootic bird-mosquito-bird cycle and occasionally transmitted to other hosts, such as equines and humans. Culex species, in particular the ubiquitous species Culex pipiens is thought to play a major vector role both in enzootic and epizootic maintenance and transmission of WNV. Introduced in Europe in recent years, since 2008 WNV has been stably circulating mainly in the Northeastern regions of Italy, although sporadic equine and/or human cases, as well as WNV infected Cx. pipiens pools, have been recorded in other Italian areas. The scope of our study was to evaluate the potential competence of some Italian populations of Cx. pipiens to transmit WNV and to assess their ability for vertical transmission of the virus. For this purpose four Italian populations, from different areas, were experimentally infected. After the infectious blood meal, fed females were monitored for 32 days to determine the length of viral extrinsic incubation period. WNV titre of infected mosquitoes was evaluated both by quantitative Real Time PCR and viral titration by Plaque Forming Units/ml (PFU/mL) in VERO cells. Potential Infection, Dissemination, Transmission rates (IR, DR, TR) were assessed by detection of the virus in body, legs plus wings and saliva of the fed females, respectively. All tested populations were susceptible to the WNV infection. The viral presence in legs and wings demonstrated the ability of WNV to disseminate in the mosquitoes. Viral RNA was detected in the saliva of tested populations. No significant differences in TR values were observed among the four studied populations. The offspring of the Cx. pipiens infected females were WNV negative. Our study addressed an important issue in the knowledge on the complex WNV-vector relationships in Italy, indicating that all Italian Cx. pipiens populations tested exhibited vector competence for WNV. Further studies should be performed in order to better clarify the role of other factors (vector density, climatic conditions, reservoir presence etc.) in order to predict where and when WNV outbreaks could occur.