Image reconstruction of oxidized cerebral cytochrome C oxidase changes from broadband near-infrared spectroscopy data.
Image reconstruction of oxidized cerebral cytochrome C oxidase changes from broadband near-infrared spectroscopy data.
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DOI：
10.1117/1.nph.4.2.021105
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发表时间：
2017-04
期刊：
影响因子：
5.3
通讯作者：
Gibson AP
中科院分区：
文献类型：
--
作者：
Brigadoi S;Phan P;Highton D;Powell S;Cooper RJ;Hebden J;Smith M;Tachtsidis I;Elwell CE;Gibson AP
在扩散光学层析成像（DOT）中，需要重叠和多距离测量来重建脑中发生的氧合血红蛋白（HHb）和脱氧血红蛋白（HHb）浓度变化的深度分辨图像。在神经血管耦合完整的假设下，这些可以被认为是大脑活动的间接测量。宽带系统还允许测量细胞色素c氧化酶（oxCCO）的氧化还原状态的变化，这可以是神经血管耦合受损时的重要生物标志物。我们使用DOT从宽带系统获得的数据中重建、和的图像。四个健康的志愿者进行了测量，同时执行视觉刺激任务（4赫兹反转棋盘）。宽带系统被配置为允许参与者的视觉皮层的32个通道的多距离和重叠测量。一个多光谱的方法来重建在视觉刺激过程中的三个生色团的浓度变化。在所有参与者的左枕叶皮层中重建了清晰和集中的激活。三发色团模型和两发色团模型（仅回收和）的残差之间的差异显示出与oxCCO相似的光谱。这些结果为进一步优化图像重建的研究奠定了基础。
In diffuse optical tomography (DOT), overlapping and multidistance measurements are required to reconstruct depth-resolved images of oxy- () and deoxy- (HHb) hemoglobin concentration changes occurring in the brain. These can be considered an indirect measure of brain activity, under the assumption of intact neurovascular coupling. Broadband systems also allow changes in the redox state of cytochrome c oxidase (oxCCO) to be measured, which can be an important biomarker when neurovascular coupling is impaired. We used DOT to reconstruct images of , , and from data acquired with a broadband system. Four healthy volunteers were measured while performing a visual stimulation task (4-Hz inverting checkerboard). The broadband system was configured to allow multidistance and overlapping measurements of the participants’ visual cortex with 32 channels. A multispectral approach was employed to reconstruct changes in concentration of the three chromophores during the visual stimulation. A clear and focused activation was reconstructed in the left occipital cortex of all participants. The difference between the residuals of the three-chromophore model and of the two-chromophore model (recovering only and ) exhibits a spectrum similar to that of oxCCO. These results form a basis for further studies aimed to further optimize image reconstruction of .