Transcriptome diversity among rice root types during asymbiosis and interaction with arbuscular mycorrhizal fungi
Transcriptome diversity among rice root types during asymbiosis and interaction with arbuscular mycorrhizal fungi
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DOI：
10.1073/pnas.1504142112
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发表时间：
2015-05-26
影响因子：
11.1
通讯作者：
Paszkowski, Uta
中科院分区：
文献类型：
--
作者：
Gutjahr, Caroline;Sawers, Ruairidh J. H.;Paszkowski, Uta
根系由具有不同发育和功能特征的不同根型组成。RT可以单独重新编程，以响应其微环境，以最大限度地提高适应性可塑性。尽管对作物改良至关重要，但对这种特定重塑的分子理解仍然有限。在这里，RT-specific转录组的成年水稻冠，大，细侧根进行了评估，揭示个体RTs之间的功能多样性的分子证据。三个水稻RTs，冠根表现出显着丰富的植物激素和次生细胞壁（SCW）代谢相关的转录本，而横向RTs表现出更大的积累与矿物运输的转录本。在自然界中，丛枝菌根（AM）共生代表了大多数根系的默认状态，并被称为修改根系结构。水稻RTs成为异质性的AM真菌定殖，大的真菌优先进入协会。然而，RT特异性转录反应AM共生冠根，尽管他们的适度的物理参与的相互作用是最明显的定量。此外，殖民冠根通过的表达谱更相关的菌根大侧比noncolonized冠根，这表明一个根本的重编程冠根字符。在这些变化中，观察到SCW转录物显著减少，这与通过质谱法测定的SCW组成的改变相关。在SCW的组合变化，植物和运输相关的转录本配置文件跨RTs表明以前被忽视的开关之间的功能关系的RTs在AM共生，根系的结构和功能的潜在影响。
Root systems consist of different root types (RTs) with distinct developmental and functional characteristics. RTs may be individually reprogrammed in response to their microenvironment to maximize adaptive plasticity. Molecular understanding of such specific remodeling-although crucial for crop improvement-is limited. Here, RT-specific transcriptomes of adult rice crown, large and fine lateral roots were assessed, revealing molecular evidence for functional diversity among individual RTs. Of the three rice RTs, crown roots displayed a significant enrichment of transcripts associated with phytohormones and secondary cell wall (SCW) metabolism, whereas lateral RTs showed a greater accumulation of transcripts related to mineral transport. In nature, arbuscular mycorrhizal (AM) symbiosis represents the default state of most root systems and is known to modify root system architecture. Rice RTs become heterogeneously colonized by AM fungi, with large laterals preferentially entering into the association. However, RT-specific transcriptional responses to AM symbiosis were quantitatively most pronounced for crown roots despite their modest physical engagement in the interaction. Furthermore, colonized crown roots adopted an expression profile more related to mycorrhizal large lateral than to noncolonized crown roots, suggesting a fundamental reprogramming of crown root character. Among these changes, a significant reduction in SCW transcripts was observed that was correlated with an alteration of SCW composition as determined by mass spectrometry. The combined change in SCW, hormone-and transport-related transcript profiles across the RTs indicates a previously overlooked switch of functional relationships among RTs during AM symbiosis, with a potential impact on root system architecture and functioning.