QTL analysis of flooding tolerance in soybean at an early vegetative growth stage
QTL analysis of flooding tolerance in soybean at an early vegetative growth stage
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DOI：
10.1111/j.1439-0523.2006.01291.x
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发表时间：
2006-12-01
期刊：
影响因子：
2
通讯作者：
Takahashi, R.
中科院分区：
文献类型：
--
作者：
Githiri, S. M.;Watanabe, S.;Takahashi, R.
大豆品种对淹水胁迫敏感，其种子产量响应于胁迫而显著降低。本研究旨在探讨营养生长早期耐淹性的遗传基础。60个重组自交系来自一个相对耐受的cv。'Misuzudaizu'和敏感的cv. 2002年和2003年在塑料大棚中种植了‘磨石豆工503’。在两叶期，将一半的盆用置于塑料容器中的水浸泡，并调节至土壤表面以上5cm。处理3周后，将盆放回温室并生长直至成熟。通过将处理植物的种子重量除以对照植物的种子重量来评价耐水淹性。2002年利用360个遗传标记进行的数量性状基因座（QTL）分析揭示了3个与耐淹性相关的QTL，分别为ft 1 ~ ft 3。在2003年发现了分子连锁群C2（ft 1）和ft 4 ~ ft 7这4个QTL。fi 1在这两年中具有较高的LOD评分（15.41和7.57），分别占总方差的49.2%和30.5%。一个大的QTL开花天数一致地观察到跨处理和年在一个类似的位置英尺1。通过与标记的相对位置比较，该成熟度基因可能对应于E1。晚熟可能赋予了一个较长的生长期从水淹胁迫恢复。
Soybean cultivars are sensitive to flooding stress and their seed yields are substantially reduced in response to the stress. This study was conducted to investigate the genetic basis of flooding tolerance at an early vegetative growth stage. Sixty recombinant inbred lines derived from a cross between a relatively tolerant cv. 'Misuzudaizu' and a sensitive cv. 'Moshidou Gong 503' were grown in pots in a vinyl plastic greenhouse in 2002 and 2003. At the two-leaf stage, half of the pots were waterlogged by water placed in plastic containers and adjusted to 5 cm above the soil Surface. After 3 weeks of treatment, the pots were returned to the greenhouse and grown until maturity. Flooding tolerance was evaluated by dividing the seed weight of the treated plants by that of the control plants. Quantitative trait loci (QTL) analysis using 360 genetic markers revealed three QTLs for flooding tolerance, ft1 to ft3 in 2002. The ft1 (molecular linkage group C2) was reproducible and an additional four QTLs, ft4 to ft7, were found in 2003. The fi1 had a high LOD score in both years (15.41 and 7.57) and accounted for 49.2% and 30.5% of the total variance, respectively. A large QTL for days to flowering was consistently observed across treatments and years at a similar position to ft1. Comparing the relative location with markers, the maturity gene probably corresponds to El. Late maturity may have conferred a longer growth period for recovery from flooding stress.