In vivo analysis of formin dynamics in the moss P. patens reveals functional class diversification
In vivo analysis of formin dynamics in the moss P. patens reveals functional class diversification
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DOI：
10.1242/jcs.233791
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发表时间：
2020-01
影响因子：
4
通讯作者：
Peter A C van Gisbergen;Shu-Zon Wu;Xiaohang Cheng;Kelli A. Pattavina;M. Bezanilla
中科院分区：
文献类型：
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作者：
Peter A C van Gisbergen;Shu-Zon Wu;Xiaohang Cheng;Kelli A. Pattavina;M. Bezanilla
摘要形成蛋白是真核生物中多种生物过程的关键肌动蛋白调节因子。由于植物和动物中存在许多formin，因此在体内确定formin的功能一直具有挑战性。我们发现，从苔藓P. patens的遗传学上不同的I类完整的膜formins（表示为For 1）在膜周转的网站富集，For 1D更紧密地与质膜比For 1A。为了探测双曲正切函数，我们生成了具有大大降低的双曲正切复杂度的formin-null线。我们发现，For 1A和For 1D有助于锚肌动蛋白附近的细胞顶端，与For 1A有助于形成细胞溶质肌动蛋白，而For 1D有助于质膜相关的肌动蛋白。在皮层，For 1A和For 1D定位于运动点和差异影响肌动蛋白动力学。我们发现，I类皮质神经元的流动性依赖于微管，只有适度的肌动蛋白，而II类神经元（表示为2）的流动性完全依赖于肌动蛋白。此外，皮质For 2A紧密相关的斑点标记的内吞膜染料FM 4 -64，和无效突变体在I类formin没有影响摄取类似的染料，FM 1 -43，这表明I类和II类formin参与不同的膜贩运途径。突出显示的文章：活细胞成像的内源性标记的formin加上检查formin的空线揭示了不同的formin差异有助于肌动蛋白在植物细胞中的形成。
ABSTRACT Formins are actin regulators critical for diverse processes across eukaryotes. With many formins in plants and animals, it has been challenging to determine formin function in vivo. We found that the phylogenetically distinct class I integral membrane formins (denoted For1) from the moss P. patens enrich at sites of membrane turnover, with For1D more tightly associated with the plasma membrane than For1A. To probe formin function, we generated formin-null lines with greatly reduced formin complexity. We found that For1A and For1D help to anchor actin near the cell apex, with For1A contributing to formation of cytosolic actin, while For1D contributes to plasma membrane-associated actin. At the cortex, For1A and For1D localized to motile puncta and differentially impacted actin dynamics. We found that class I cortical formin mobility depended on microtubules and only moderately on actin, whereas class II formin (denoted For2) mobility solely depended on actin. Moreover, cortical For2A tightly correlated with the puncta labeled by the endocytic membrane dye FM4-64, and null mutants in class I formins did not affect uptake of a similar dye, FM1-43, suggesting that class I and II formins are involved in distinct membrane trafficking pathways. Highlighted Article: Live-cell imaging of endogenously tagged formins coupled with examination of formin-null lines reveals that phylogenetically distinct formins differentially contribute to actin formation in plant cells.