Recombinant vesicular stomatitis virus expressing the spike protein of genotype 2b porcine epidemic diarrhea virus: A platform for vaccine development against emerging epidemic isolates
Recombinant vesicular stomatitis virus expressing the spike protein of genotype 2b porcine epidemic diarrhea virus: A platform for vaccine development against emerging epidemic isolates
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DOI：
10.1016/j.virol.2019.05.009
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发表时间：
2019-07-01
期刊：
影响因子：
3.7
通讯作者：
Sun, Tao
中科院分区：
文献类型：
--
作者：
Ke, Yong;Yu, Dayi;Sun, Tao
新发猪流行性腹泻病毒（PEDVs）自2010年以来造成了巨大的经济损失，G2b是全球流行的基因型。考虑到新发PEDV在细胞培养中难以分离，且体外传代难以保持分离物的感染性，本研究以高度减毒的重组水疱性口炎病毒（rVSV(MT)）为载体，表达PEDV刺突(S)蛋白，旨在开发一种针对G2b病毒的亚单位疫苗。一个缺失了19个胞质结构域氨基酸的S蛋白可以被整合到VSV颗粒中，高效地生成rVSV(MT) （VSVMT-S-Delta 19）。我们的研究结果表明，VSVMT-S-Delta 19可以通过肌肉注射而不是鼻内免疫有效地诱导猪对pedv的特异性免疫。值得注意的是，接种VSV (MT)-S-Delta 19的母猪对仔猪的G2b强毒PEDV攻击具有保护性的乳原免疫。因此，重组VSVMT可能是制备PEDV亚单位疫苗的一个有希望的平台。
Emerging porcine epidemic diarrhea viruses (PEDVs) have caused large economic losses since 2010, and G2b is the prevalent globally epidemic genotype. Given the fastidious isolation of emerging PEDV in cell culture and difficulties in retaining the isolate infectivity upon further in vitro passage, highly attenuated recombinant vesicular stomatitis virus (rVSV(MT)) was used as a vector to express the PEDV spike (S) protein, aiming to develop a subunit vaccine against G2b viruses. An S protein with 19 of its cytoplasmic domain amino acids deleted could be incorporated into VSV particles, generating rVSV(MT) (VSVMT-S-Delta 19) with high efficiency. Our results suggest that VSVMT-S-Delta 19 could effectively induce PEDV-specific immunity in pigs via intramuscular, but not intranasal, immunization. Notably, immunizations of sows with VSV (MT)-S-Delta 19 provided protective lactogenic immunity against a virulent G2b PEDV challenge in piglets. Consequently, recombinant VSVMT may be a promising platform for preparing a subunit vaccine against PEDV.