COMMON INDUCTION AND REGULATION OF BIPHENYL, XYLENE TOLUENE, AND SALICYLATE CATABOLISM IN PSEUDOMONAS-PAUCIMOBILIS
COMMON INDUCTION AND REGULATION OF BIPHENYL, XYLENE TOLUENE, AND SALICYLATE CATABOLISM IN PSEUDOMONAS-PAUCIMOBILIS
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DOI：
10.1128/jb.154.3.1356-1362.1983
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发表时间：
1983-01-01
影响因子：
3.2
通讯作者：
CHAKRABARTY, AM
中科院分区：
文献类型：
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作者：
FURUKAWA, K;SIMON, JR;CHAKRABARTY, AM
从土壤中分离到一株能利用联苯的少动假单胞菌Q1。该菌株生长在取代联苯和水杨酸盐、二甲苯或甲苯或两者（二甲苯/甲苯）和取代苯甲酸盐上。有证据表明，联苯，二甲苯/甲苯和水杨酸盐的催化剂是由一个共同的单位在这种菌株。联苯、二甲苯/甲苯和水杨酸盐的催化剂是相互关联的，因为苯甲酸盐和甲苯甲酸盐是联苯和二甲苯/甲苯的常见代谢中间体，而水杨酸盐是由2-羟基联苯（邻苯基苯酚）产生的。当细胞生长在联苯、二甲苯/甲苯或水杨酸盐上时，联苯、二甲苯/甲苯和水杨酸盐降解途径的所有氧化酶都被诱导。P. paucimobilis Q1细胞显示出2，3-二羟基联苯和邻苯二酚的间位裂解酶的诱导。联苯阴性衍生物的菌株Q1的二甲苯/甲苯和水杨酸盐同时呈现阴性;这种衍生物的联苯阳性特征的逆转总是导致二甲苯/甲苯和水杨酸盐阳性表型。以苯甲酸盐作为唯一C源的少动毕赤酵母Q1细胞的生长仅允许邻位途径酶的诱导，这表明联苯、二甲苯/甲苯或水杨酸盐特异性地诱导用于这些化合物的氧化降解的Meta途径酶。
P. paucimobilis strain Q1 capable of utilizing biphenyl was isolated from soil. This strain grew on substituted biphenyls and on salicylate, xylene or toluene or both (xylene/toluene), and substituted benzoates. Evidence is presented that the catabolism of biphenyl, xylene/toluene and salicylate is regulated by a common unit in this strain. The catabolism of biphenyl, xylene/toluene and salicylate is interrelated, since benzoate and toluate are common metabolic intermediates of biphenyl and xylene/toluene and salicylate is produced from 2-hydroxybiphenyl (o-phenylphenol). All the oxidative enzymes of the biphenyl, xylene/toluene and salicylate degradative pathways were induced when the cells were grown on biphenyl, xylene/toluene or salicylate. P. paucimobilis Q1 cells showed induction of the meta-cleavage enzymes of 2,3-dihydroxybiphenyl and catechol. Biphenyl-negative derivatives of strain Q1 were simultaneously rendered xylene/toluene and salicylate negative; reversion to the biphenyl-positive character of such derivatives invariably led to a xylene/toluene- and salicylate-positive phenotype. Growth of P. paucimobilis Q1 cells with benzoate as sole C source allowed the induction of only the ortho pathway enzymes, suggesting that biphenyl, xylene/toluene or salicylate specifically induced the meta pathway enzymes for the oxidative degradation of these compounds.