Development-on-chip: in vitro neural tube patterning with a microfluidic device.
Development-on-chip: in vitro neural tube patterning with a microfluidic device.
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DOI：
10.1242/dev.126847
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发表时间：
2016-06-01
期刊：
影响因子：
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通讯作者：
Smith RL
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文献类型：
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作者：
Demers CJ;Soundararajan P;Chennampally P;Cox GA;Briscoe J;Collins SD;Smith RL
胚胎发生是一个高度调控的过程，在这个过程中，可溶性线索的精确空间和时间释放指导多能干细胞向特化成体细胞类型的离散群体分化。在脊髓中，神经祖细胞通过附近组织中心（如底板和近轴中胚层）释放的介质的作用，定向分化为成体神经元。这些信号结合在一起，形成时空扩散景观，精确地调节中枢神经系统（CNS）的发育。目前，对这些信号因子的体内和体外研究存在一些固有的模糊性。体外方法是首选的，因为它们增强了实验的清晰度，但往往缺乏生物真实性所需的技术复杂性。在本文中，我们提出了一个多功能的微流控平台，能够模拟在神经管发育过程中发现的体内空间和时间化学环境。发育形态因子的同时对立和/或正交梯度可以维持，从而产生类似于在体内观察到的神经管模式。摘要：微流控装置模拟体内神经管发育的时空环境，使体外干细胞形成神经管的空间组织正确。
Embryogenesis is a highly regulated process in which the precise spatial and temporal release of soluble cues directs differentiation of multipotent stem cells into discrete populations of specialized adult cell types. In the spinal cord, neural progenitor cells are directed to differentiate into adult neurons through the action of mediators released from nearby organizing centers, such as the floor plate and paraxial mesoderm. These signals combine to create spatiotemporal diffusional landscapes that precisely regulate the development of the central nervous system (CNS). Currently, in vivo and ex vivo studies of these signaling factors present some inherent ambiguity. In vitro methods are preferred for their enhanced experimental clarity but often lack the technical sophistication required for biological realism. In this article, we present a versatile microfluidic platform capable of mimicking the spatial and temporal chemical environments found in vivo during neural tube development. Simultaneous opposing and/or orthogonal gradients of developmental morphogens can be maintained, resulting in neural tube patterning analogous to that observed in vivo. Summary: A microfluidic device mimics the spatial and temporal environment of neural tube development in vivo and enables the correct spatial organization of neural tube formation from stem cells in vitro.