PEI1, an embryo-specific zinc finger protein gene required for heart-stage embryo formation in Arabidopsis
PEI1, an embryo-specific zinc finger protein gene required for heart-stage embryo formation in Arabidopsis
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DOI：
10.1105/tpc.10.3.383
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发表时间：
1998-03-01
期刊：
影响因子：
11.6
通讯作者：
Thomas, TL
中科院分区：
文献类型：
--
作者：
Li, ZS;Thomas, TL
我们使用虚拟减法，一种新的基因分离策略，分离出几个在拟南芥胚胎中表达的感兴趣的基因。这些基因已显示出生物学特性或有可能参与重要的生物过程。通过虚拟减法分离出的一个基因是PEI。它编码一种含有与许多动物和真菌转录因子相关的Cys(3)His锌指结构域的蛋白质。原位杂交结果表明，PEI1在胚芽中从球形到子叶晚期都有表达。表达PEI1反义基因的转基因拟南芥植株产生白色种子，胚胎发育不经过心脏期。异常胚不能形成子叶，但胚根是正常的。异常胚胎不变绿，参与光形态发生的基因表达急剧减弱。在培养中，异常胚不能形成真叶，但根的形成明显正常。这些结果表明，PEI1是一种胚胎特异性转录因子，在拟南芥胚胎发生过程中起重要作用，主要在胚胎的顶端区域起作用。
We used virtual subtraction, a new gene isolation strategy, to isolate several genes of interest that are expressed in Arabidopsis embryos. These genes have demonstrated biological properties or have the potential to be involved in important biological processes. One gene isolated by virtual subtraction is PEI. It encodes a protein containing a Cys(3)His zinc finger domain associated with a number of animal and fungal transcription factors. In situ hybridization results showed that PEI1 is expressed throughout the embryo from globular to late cotyledon stage. Transgenic Arabidopsis plants expressing a PEI1 antisense gene produced white seeds in which embryo development did not progress through heart stage. Aberrant embryos failed to form cotyledons, but the embryonic root appeared to be normal. Aberrant embryos did not turn green, and the expression of genes involved in photomorphogenesis was drastically attenuated. In culture, aberrant embryos did not form true leaves, but root formation was apparently normal. These results suggest that PEI1 is an embryo-specific transcription factor that plays an important role during Arabidopsis embryogenesis, functioning primarily in the apical domain of the embryo.