An Annexin V-FITC-Propidium Iodide-Based Method for Detecting Apoptosis in a Non-Small Cell Lung Cancer Cell Line
An Annexin V-FITC-Propidium Iodide-Based Method for Detecting Apoptosis in a Non-Small Cell Lung Cancer Cell Line
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DOI：
10.1007/978-1-0716-1278-1_17
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发表时间：
2021-01-01
期刊：
影响因子：
5.3
通讯作者：
Panda, Amulya K.
中科院分区：
文献类型：
--
作者：
Kumar, Robin;Saneja, Ankit;Panda, Amulya K.
膜联蛋白 V 和碘化丙啶染色广泛用于通过细胞凋亡确定细胞死亡。在存在 Ca2+ 离子的情况下，膜联蛋白 V 对磷脂酰丝氨酸具有很强的结合亲和力，磷脂酰丝氨酸是一种膜磷脂，在细胞凋亡过程中从细胞膜内侧易位至外侧。另一方面，碘化丙啶具有DNA结合能力，只能进入坏死或凋亡晚期的细胞。本章介绍了使用膜联蛋白 V 和碘化丙啶染料检测非小细胞肺癌细胞系细胞凋亡的常用方法。我们描述了用二氢青蒿素 (DHA) 处理的 A549 肺癌细胞系中不同阶段细胞凋亡的检测。这种细胞凋亡检测方法可用于确定不同种类的药物对培养的癌细胞系的疗效。
Annexin V and propidium iodide staining is widely used for determining the cellular death through apoptosis. In the presence of Ca2+ ions, annexin V has a strong binding affinity for phosphatidylserine, a membrane phospholipid that during apoptosis is translocated from the inner side of the cell membrane to its outer side. On the other hand, propidium iodide has ability for DNA binding and it can only enter into necrotic or late apoptotic cells. This chapter describes a commonly used method for detection of apoptosis in a non-small cell lung cancer cell line using annexin V and propidium iodide dye. We describe the detection of different stages of apoptosis in the A549 lung cancer cell line treated with dihydroartemisinin (DHA). This apoptosis detection method can be used to determine the efficacy of different kinds of drugs on cultured cancer cell lines.