Enhancement of amygdalin activated with β-D-glucosidase on HepG2 cells proliferation and apoptosis
Enhancement of amygdalin activated with β-D-glucosidase on HepG2 cells proliferation and apoptosis
复制标题
DOI：
10.1016/j.carbpol.2012.05.073
复制
发表时间：
2012-09-01
影响因子：
11.2
通讯作者：
Xia, Wei
中科院分区：
文献类型：
--
作者：
Zhou, Cunshan;Qian, Lichun;Xia, Wei
在HepG 2细胞中检测苦杏仁苷和β-D-葡萄糖苷酶激活的生长抑制和诱导凋亡的细胞活性。MTT法检测各组样品对HepG 2细胞增殖的影响均呈剂量和时间效应关系。单独苦杏仁苷和β-D-葡萄糖苷酶激活对HepG 2细胞增殖48 h的IC 50分别为458.10 mg/mL和3.2 mg/mL。采用AO/EB（吖啶橙子/溴化乙锭）荧光染色法和Annexin V-FITC/PI染色流式细胞仪细胞周期分析法检测凋亡细胞。随着苦杏仁甙浓度的增加和作用时间的延长，细胞凋亡率增加。与对照组比较，差异有显著性（P < 0.01）。总之，这些发现表明苦杏仁苷没有强的抗HepG 2活性;然而，用β-D-葡糖苷酶活化的苦杏仁苷的成分具有更高且有效的抗HepG 2活性。因此，这表明该组合策略可适用于治疗具有更高活性的肿瘤。(C)2012爱思唯尔有限公司保留所有权利。
The growth inhibition and induction of apoptosis brought by amygdalin and activated with beta-D-glucosidase were tested for cytoactivity in HepG2 cells. The MTT viability assay showed that all samples had effects on HepG2 proliferation in dose and time response manners. IC50 of stand-alone amygdalin and activation with beta-D-glucosidase on the proliferation of HepG2 cells for 48 h were 458.10 mg/mL and 3.2 mg/mL, respectively. Moreover, apoptotic cells were determined by AO/EB (acridine orange/ethidium bromide) fluorescent staining method and Annexin V-FITC/PI staining flow cytometry cell cycle analysis. With increasing of amygdalin concentration and the incubation time, the apoptotic rate was heightened. Compared with the control, there was significant difference (p < 0.01). Together, these findings indicate that amygdalin had no strong anti-HepG2 activity; however the ingredients of amygdalin activated with beta-D-glucosidase had a higher and efficient anti-HepG2 activity. It was therefore suggested that this combination strategy may be applicable for treating tumors with a higher activity. (C) 2012 Elsevier Ltd. All rights reserved.