A unique transcription factor for the A alpha fibrinogen gene is related to the mitochondrial single-stranded DNA binding protein P16.
A unique transcription factor for the A alpha fibrinogen gene is related to the mitochondrial single-stranded DNA binding protein P16.
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A α 纤维蛋白原基因的独特转录因子与线粒体单链 DNA 结合蛋白 P16 相关。
DOI：
10.1021/bi970946u
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发表时间：
1997
期刊：
影响因子：
--
通讯作者：
Fuller,GM
中科院分区：
文献类型：
--
作者：
Liu,Z;Fuentes,NL;Jones,SA;Hagood,JS;Fuller,GM
尽管白介素6刺激肝细胞可以诱导纤维蛋白原的表达，但这种调节的分子基础在很大程度上仍不清楚。最近对Aα纤维蛋白原基因启动子的检测发现了一种被称为Aα核心蛋白的蛋白质，它与IL-6反应元件构成结合[Liu，Z.&Fuller，G.M.(1995)J.Biol.Chem.270,7580−7586]。这项目前的研究提供了这种调节蛋白的进一步特征。结果表明： (I)ADNA核心蛋白与线粒体单链α结合蛋白P16具有相似的相对分子质量和相同的N-末端序列。(Ii)ADNA核心蛋白和P16在α结合偏好和抗原性方面具有相似的特征。(3)P16基因在肝癌细胞株HEP G2和HEP 3B中的过表达增强了IL-6诱导的Aα纤维蛋白原的表达。这些结果表明，Aα核心蛋白与P16密切相关，并参与IL-6调控的Aα纤维蛋白原转录。
Although stimulation of hepatic cells with interleukin-6 induces the expression of fibrinogen, the molecular basis for this regulation remains largely uncharacterized. A recent examination of the Aα fibrinogen gene promoter identified a protein, termed the Aα-core protein, that bound constitutively to the IL-6 response element [Liu, Z. & Fuller, G. M. (1995)J.Biol.Chem.270, 7580−7586]. This current study provides further characterization of this regulatory protein. The data presented show the following: (i) The Aα-core protein has a similar molecular weight and identical N-terminal sequence to that of the mitochondrial single-stranded DNA binding protein P16. (ii) The Aα-core protein and P16 have similar characteristics in terms of DNA binding preference and antigenic properties. (iii) Overexpression of P16 gene in the hepatoma cell lines Hep G2 and Hep 3B enhances the IL-6-induced expression of Aα fibrinogen. These results demonstrate that the Aα-core protein is closely related to P16 and involved in the IL-6-regulated transcription of Aα fibrinogen.