Biodefense function of omental milky spots through cell adhesion molecules and leukocyte proliferation
Biodefense function of omental milky spots through cell adhesion molecules and leukocyte proliferation
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DOI：
10.1007/s00441-002-0636-6
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发表时间：
2002-12-01
影响因子：
3.6
通讯作者：
Sasaki, K
中科院分区：
文献类型：
--
作者：
Cui, L;Johkura, K;Sasaki, K
为了评估腹膜腔大网膜的免疫功能，通过免疫电子显微镜研究了正常和脂多糖（LPS）刺激的小鼠中大网膜乳斑中的间皮细胞和白细胞上细胞粘附分子（CAM）的定位。即使在正常稳定状态下，乳白色斑点的圆顶状间皮细胞中也存在大量白细胞。白细胞整合素 LFA-1、Mac-1 和 VLA-4 优先定位于巨噬细胞和淋巴细胞的微绒毛和褶边。乳白色斑点的间皮细胞显示出比其他网膜区域更高的 ICAM-1 水平，并且在气孔中检测到纤连蛋白。 LPS刺激后乳白色斑点中的增殖细胞核抗原（PCNA）阳性细胞增加，白细胞数量明显增加。间皮细胞含有新限制于微绒毛的 VCAM-1 和数量不断增加的 ICAM-1。这些结果表明，由于粘附分子的存在和活跃的细胞增殖，大网膜乳斑是白细胞迁移和腹膜白细胞供应的活跃部位。增殖活性白细胞和从血管迁移的白细胞通过 VLA-4 和纤连蛋白的相互作用穿过气孔，由于 ICAM-I/VCAM-1 和整合素之间的相互作用而粘附到活化的间皮细胞表面的微绒毛，并渗出到腹膜腔中。在 LPS 刺激的小鼠的乳白色斑点中看到的大部分白细胞渗出和粘附可能归因于细胞增殖以及 ICAM-1 和 VCAM-1 量的增加。
To evaluate the immunological functions of the greater omentum in the peritoneal cavity, the localization of cell adhesion molecules (CAMs) on mesothelial cells and leukocytes in the omental milky spots were studied in normal and lipopolysaccharide (LPS)-stimulated mice by means of immunoelectron microscopy. The milky spots featured numerous leukocytes among the dome-shaped mesothelial cells, even in the normal stable state. Leukocyte integrins LFA-1, Mac-1, and VLA-4 were preferentially localized to microvilli and ruffles of macrophages and lymphocytes. The mesothelial cells of the milky spots showed higher ICAM-1 levels than did those of other omental regions, and fibronectin was detected in the stomata. The number of leukocytes markedly increased following an increase in proliferating cell nuclear antigen (PCNA)-positive cells in the milky spots after LPS stimulation. The mesothelial cells contained VCAM-1 newly restricted to the microvilli and increasing amounts of ICAM-1. These results show that the omental milky spots are active sites for leukocyte migration and peritoneal leukocyte supply because of the presence of adhesion molecules and active cell proliferation. Proliferative active leukocytes and those that have migrated from vessels pass through the stomata via an interaction of VLA-4 and fibronectin, adhere to the microvilli of the activated mesothelial cell surface as the result of an interaction between ICAM-I/VCAM-1 and integrins, and exude into the peritoneal cavity. Much of the exudation and adhesion of leukocytes seen in the milky spots of LPS-stimulated mice may be attributable to an increase in cell proliferation and in the amounts of ICAM-1 and VCAM-1.